Human Anti-CD3 Recombinant Antibody (clone UCHT-1); scFv Fragment (CAT#: HPAB-M0467-YC-S(P))

Figure 1 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in SDS-PAGE

SDS-PAGE analysis of HPAB-M0467-YC-S(P) in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced and non-reduced conditions, the purified proteins migrate as about 25-35 kDa which is consistent with theoretical size.

Figure 2 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in SEC-HPLC

The purity of HPAB-M0467-YC-S(P) was analyzed by SEC-HPLC. The result shows that the scFv fragment forms a small number of dimers.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in WB

Western blot analysis of HPAB-M0467-YC-S(P) was performed by loading human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for one hour. Membranes were probed with HPAB-M0467-YC-S(P) (2 ng/μL) and HRP mouse anti-His tag as a secondary antibody (1: 2000) in turn. Chemiluminescent detection was performed.

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

Figure 4 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in FACS

Flow cytometry analysis of Jurkat cells incubated with anti-human CD3 recombinant antibody cat# HPAB-M0467-YC-S(P). Jurkat cells were stained with HPAB-M0467-YC-S(P) (blue histogram, TE232382) or isotype control antibody (yellow histogram), followed by APC-conjugated goat anti-His tag secondary antibody. The used concentration of HPAB-M0467-YC-S(P) was 10 µg per 5 x 10^6 cells.