Mouse Anti-CD48 Recombinant Antibody (clone OX45) (CAT#: FAMAB-1650CQ)

Figure 1 Effect of MRC OX-45 and MRC OX-46 MAbs on MLR proliferation.

HO lymph node cells were incubated with 5 x 105 irradiated F1 spleen cells for 4 days in RPMI medium containing 5% DA serum. The cells were cultured in 0.2 ml/well in sterile microtitre plates. Monoclonal antibodies were added to the culture at the beginning of the incubation as pure IgG and/or F(ab')2 for MRC OX-45 antibody. Cultures were labelled with 0.5 uCi [3H]thymidine 18 hr before the end of the incubation period, and the cells were harvested on glass fibre filters. Data are means and ranges of quadruplicate determinations. Cells were cultured with MRC OX-46 IgG (●) MRC OX-45F(ab')2 (○) or MRC OX-l IgG included as a control (△). The dose-response curve for MRC OX-45 IgG (not shown) was similar to those for MRC OX-46 IgG and MRC OX-45 F(ab')2.

Arvieux, J., Jefferies, W. A., Paterson, D. J., Williams, A. F., & Green, J. R. (1986). Monoclonal antibodies against a rat leucocyte antigen block antigen-induced T-cell responses via an effect on accessory cells. Immunology, 58(3), 337.

Figure 2 Time-course of IL-2 production in MLR in the presence of MRC OX-45 IgG.

Primary MLR between HO lymph node cells(2.5 x 105) and (HO x HO.B2) F, spleen cells (5 x 105) were incubated for 5 days in RPMI containing 5% DA serum with (●) or without (△)MRC OX-45 IgG added at the beginning of the culture. Supernatants were collected daily and IL-2 activity was assayed on Con A-activated blasts. One U/ml of IL-2 activity corresponded to the dilution that produced 10% maximal proliferation of target cells.

Arvieux, J., Jefferies, W. A., Paterson, D. J., Williams, A. F., & Green, J. R. (1986). Monoclonal antibodies against a rat leucocyte antigen block antigen-induced T-cell responses via an effect on accessory cells. Immunology, 58(3), 337.