Rabbit Anti-CD63 Recombinant Antibody (clone 2E5) (CAT#: ZG-0593J)

Figure 1 Rabbit Anti-CD63 Antibody (ZG-0593J) in IHC

IHC image of ZG-0593J diluted at 1:155 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 2 Rabbit Anti-CD63 Antibody (ZG-0593J) in IHC

IHC image of ZG-0593J diluted at 1:155 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-CD63 Antibody (ZG-0593J) in IF

Immunofluorescence staining of A549 cells with ZG-0593J at 1:51, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).