Rabbit Anti-CD63 Recombinant Antibody (clone 2E5)
CAT#: ZG-0593J
This product is a rabbit antibody that recognizes CD63.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-CD63 Antibody (ZG-0593J) in IHC
IHC image of ZG-0593J diluted at 1:155 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 2 Rabbit Anti-CD63 Antibody (ZG-0593J) in IHC
IHC image of ZG-0593J diluted at 1:155 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-CD63 Antibody (ZG-0593J) in IF
Immunofluorescence staining of A549 cells with ZG-0593J at 1:51, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
❮
❯
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73506/1798_B5_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/ihc_selected.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_4_6.jpg
Normal Tissue
Figure 8 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26880_B_6_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135404-CD63
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human CD63
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD63
- Species Reactivity
- Human
- Clone
- 2E5
- Applications
- ELISA, IHC, IF
- Conjugate
- Uconjugated
- Related Disease
- Cardiovascular
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.
IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- MLA1; ME491; LAMP-3; OMA81H; TSPAN30; CD63 antigen; CD63 antigen (melanoma 1 antigen); granulophysin; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; ocular melanoma-associated antigen; tetraspanin-30; tspan-30
- Gene ID
- 967
- UniProt ID
- P08962
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0593J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CD63"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0575 | Hi-Affi™ Rabbit Anti-CD63 Recombinant Antibody (clone DS575AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0071-YJ-S(P) | Human Anti-CD63 Recombinant Antibody (clone Hu33-11A); scFv Fragment | FC, ELISA, IHC | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0071-YJ-F(E) | Human Anti-CD63 Recombinant Antibody (clone Hu33-11A); Fab Fragment | FC, ELISA, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0127J | Mouse Anti-CD63 Recombinant Antibody (ZG-0127J) | IHC-P, IF | Mouse IgG |
| ZG-0128J | Mouse Anti-CD63 Recombinant Antibody (ZG-0128J) | IHC-P, IF | Mouse IgG |
| VS3-FY308 | Recombinant Rabbit Anti-Cd63 Antibody (clone R06-4G3) | WB, IHC-P | Rabbit IgG |
| VS3-FY309 | Recombinant Mouse Anti-CD63 Antibody (clone 8H1-8H6-5G7) | IHC-P | Mouse IgG2a |
| VS3-FY310 | Recombinant Rabbit Anti-CD63 Antibody (clone R08-7C9) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY64 | CytoStream™ Mouse Anti-CD63 Recombinant Antibody (clone AD1) | FC, IHC, IP | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY369 | Anti-CD63 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1207 | Anti-Mouse CD63 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1208 | Anti-Rat CD63 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC180 | Recombinant Anti-CD63 Vesicular Antibody, EV Displayed (VS-0425-YC180) | ELISA, FC, Inhib, Cell-uptake |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, WB, FC
Application: WB, ELISA, IHC, IP, SPR, FuncS
Application: FC, IA, IF, IP, IHC, FuncS
Application: ELISA, Inhib, FC, Neut
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
