Anti-CD74 Antibody Prodrug, Protease Activated (VS-1025-YC195)
CAT#: VS-1025-YC195
The Anti-CD74 Antibody Prodrug, Protease Activated (VS-1025-YC195) is an antibody prodrug comprised of a recombinant human antibody targeting human CD74, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to CD74 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to CD74 effectively. CD74 (MHC Class II invariant chain) is a cell-surface protein that is overexpressed in various B-cell malignancies and solid tumors. Its high expression and internalization properties make it an attractive target for antibody-drug conjugates that deliver a toxic payload specifically to the cancer cell.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human CD74
- Target
- CD74
- Species Reactivity
- Human, Monkey
- Isotype
- IgG
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- CD74 (CD74 Molecule) is a Protein Coding gene. Diseases associated with CD74 include Undifferentiated Pleomorphic Sarcoma and Mantle Cell Lymphoma. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System. Gene Ontology (GO) annotations related to this gene include identical protein binding and amyloid-beta binding.
- Alternative Names
- CD74 Molecule; CD74 Molecule; HLA-DR Antigens-Associated Invariant Chain; MHC HLA-DR Gamma Chain; HLA-DR-Gamma; DHLAG
- Gene ID
- 972
- UniProt ID
- P04233
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Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-466-VHH | Recombinant Anti-Human CD74 VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1542z | Mouse Anti-CD74 Recombinant Antibody (clone 24B6) | FC, IF, IHC, IP, FuncS | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-139CL | Human Anti-CD74 Recombinant Antibody (TAB-139CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0004CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0004CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0005CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0005CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0006CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0006CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0007CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0007CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0569CL | Human Anti-CD74 Recombinant Antibody (TAB-0569CL) | IHC, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0570CL | Human Anti-CD74 Recombinant Antibody (TAB-0570CL) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0571CL | Human Anti-CD74 Recombinant Antibody (TAB-0571CL) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0572CL | Human Anti-CD74 Recombinant Antibody (TAB-0572CL) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0454MZ | Recombinant Mouse Anti-Human CD74 Antibody (clone MO3 (Xpsltipq JW) (pvs eftjhobujpo 349)) | ICC, IHC-Fr, IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1595 | PE-DRB*01:01/Human CD74 (KPVSKMRMATPLLMQALPM) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1598 | PE-DRB*01:01/Human CD74 (KMRMATPLLMQALPM) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1712 | PE-DRB*01:01/Human CD74 (MKMRMATPLLMQALPM) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1596 | APC-DRB*01:01/Human CD74 (KPVSKMRMATPLLMQALPM) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1599 | APC-DRB*01:01/Human CD74 (KMRMATPLLMQALPM) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-408CQ | Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC, IHC-Fr, IHC-P, ICC, IP, WB, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0579 | Hi-Affi™ Rabbit Anti-CD74 Recombinant Antibody (clone DS579AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0316-CN | Rabbit Anti-CD74 Recombinant Antibody (clone JF40-10) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY | Human Anti-CD74 Recombinant Antibody (HPAB-0388-FY) | FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (HPAB-0388-FY-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-F(E) | Human Anti-CD74 Recombinant Antibody; Fab Fragment (HPAB-0388-FY-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1081CQ | Mouse Anti-CD74 Recombinant Antibody (clone YAML 555.6) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY55 | AbPlus™ Anti-CD74 Magnetic Beads (4ALN2) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC321 | AbPlus™ Anti-CD74 Magnetic Beads (VS-0724-YC321) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY110 | Mouse Anti-NHP CD74 Recombinant Antibody (clone LN-2) | WB, IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY71 | CytoStream™ Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY83 | Human Anti-CD74 (clone LL1) scFv-Fc Chimera | FC, ELISA, Cyt | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC330 | Recombinant Anti-CD74 Vesicular Antibody, EV Displayed (VS-0425-YC330) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1217 | Anti-CD74 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1218 | Anti-Mouse CD74 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC68 | SmartAb™ Recombinant Anti-CD74 pH-dependent Antibody (VS-0825-YC68) | FC | Human IgG |
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