Human Anti-CD74 (clone LL1) scFv-Fc Chimera
CAT#: VS-0325-FY83
The anti-CD74 scFv-Fc antibody targets CD74, a molecule involved in antigen presentation and immune regulation. It can modulate CD74 signaling, potentially affecting immune responses and autoimmunity. The scFv-Fc format provides high specificity and prolonged half-life, enhancing its utility in research and therapeutic applications.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human CD74
- Species Reactivity
- Human
- Clone
- LL1
- Applications
- Flow Cytometry, ELISA, Cytotoxicity
- Related Disease
- Autoimmune disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Cytotoxicity: 5 μg/mL
Target
- Alternative Names
- CD74 Molecule; CD74 Antigen (Invariant Polypeptide Of Major Histocompatibility Complex, Class II Antigen-Associated); CD74 Molecule, Major Histocompatibility Complex, Class II Invariant Chain; HLA-DR Antigens-Associated Invariant Chain; Gamma Chain Of Class II Antigens; Ia-Associated Invariant Chain; MHC HLA-DR Gamma Chain; HLA-DR-Gamma; DHLAG
- Gene ID
- 972
- UniProt ID
- P04233
- Long Name
- CD74 Molecule
- Cellular Localization
- Cell membrane; Endoplasmic reticulum; Endosome; Golgi apparatus; Lysosome; Membrane
- Function
- CD74, also known as the invariant chain, is a transmembrane protein that plays a key role in antigen presentation by MHC class II molecules. Within antigen-presenting cells (APCs), CD74 binds to MHC class II molecules in the endoplasmic reticulum, preventing the premature binding of endogenous peptides. It then guides MHC class II molecules to endosomal compartments, where it is cleaved, allowing for the loading of antigenic peptides. CD74 also functions as a receptor for macrophage migration inhibitory factor (MIF), influencing cell signaling and inflammation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone LL1"
- CAT
- Product Name
See other products for "CD74"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-466-VHH | Recombinant Anti-Human CD74 VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| TAB-0004CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0004CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| TAB-0005CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0005CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| TAB-0006CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0006CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| TAB-0007CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0007CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0569CL | Human Anti-CD74 Recombinant Antibody (TAB-0569CL) | IHC, WB | Human IgG |
| TAB-0570CL | Human Anti-CD74 Recombinant Antibody (TAB-0570CL) | ELISA, FC | Human IgG |
| TAB-0571CL | Human Anti-CD74 Recombinant Antibody (TAB-0571CL) | ELISA, FC | Human IgG |
| TAB-0572CL | Human Anti-CD74 Recombinant Antibody (TAB-0572CL) | ELISA, FC | Human IgG |
| TAB-0573CL | Human Anti-CD74 Recombinant Antibody (TAB-0573CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-408CQ | Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC, IHC-Fr, IHC-P, ICC, IP, WB, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0579 | Hi-Affi™ Rabbit Anti-CD74 Recombinant Antibody (clone DS579AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (HPAB-0388-FY-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-F(E) | Human Anti-CD74 Recombinant Antibody; Fab Fragment (HPAB-0388-FY-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1081CQ | Mouse Anti-CD74 Recombinant Antibody (clone YAML 555.6) | ELISA | Mouse IgG1 |
| VS3-XY404 | Mouse Anti-CD74 Recombinant Antibody (clone 2D1B11) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY405 | Mouse Anti-CD74 Recombinant Antibody (clone 2D1B3) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY406 | Mouse Anti-CD74 Recombinant Antibody, FITC (clone 4ALN2) | FC, IF | Mouse IgG1 |
| VS3-FY316 | Recombinant Mouse Anti-CD74 Antibody (clone 1B7-6A7-5G10) | IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY55 | AbPlus™ Anti-CD74 Magnetic Beads (4ALN2) | IP, Protein Purification | |
| VS-0724-YC321 | AbPlus™ Anti-CD74 Magnetic Beads (VS-0724-YC321) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY110 | Mouse Anti-NHP CD74 Recombinant Antibody (clone LN-2) | WB, IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY71 | CytoStream™ Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC330 | Recombinant Anti-CD74 Vesicular Antibody, EV Displayed (VS-0425-YC330) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1217 | Anti-CD74 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1218 | Anti-Mouse CD74 Immunohistochemistry Kit | IHC |
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