Rabbit Anti-CDC25C Recombinant Antibody (clone R02-6D2)
CAT#: VS3-XY3302
This antibody is a recombinant rabbit antibody targeting the CDC25C (Cell division cycle 25C), which functions as a dosage-dependent inducer in mitotic control. It reacts with human CDC25C. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66991/1250_B5_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000158402-CDC25C
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDC25C
- Species Reactivity
- Human
- Clone
- R02-6D2
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Ataxia-Telangiectasia; Colorectal Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- CDC25; PPP1R60
- Gene ID
- 995
- UniProt ID
- P30307
- Long Name
- Cell division cycle 25C
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase.
- Protein Refseq
- XP_006714802.1; XP_005272202.1
- Function
- Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CDC25C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2469MZ | Recombinant Mouse Anti-Human CDC25C Antibody | ELISA, IHC-P, WB | Mouse antibody |
| MRO-1764-CN | Rabbit Anti-CDC25C Polyclonal Antibody (MRO-1764-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0400F | Mouse Anti-CDC25C Recombinant Antibody (ZG-0400F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0603J | Rabbit Anti-CDC25C Recombinant Antibody (clone 3E6) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-WK218 | Rabbit Anti-CDC25C Recombinant Antibody (clone JA10-49) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0110MZ | Chicken Anti-CDC25c Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0601 | Hi-Affi™ Rabbit Anti-CDC25C Recombinant Antibody (clone DS601AB) | ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1259 | Anti-CDC25C Immunohistochemistry Kit | IHC |
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