Rabbit Anti-CDC42 Recombinant Antibody (clone 3C3) (CAT#: ZG-0101U)
Rabbit anti-CDC42 antibody (clone 3C3) displayed high specificity against the CDC42 protein of Human and Mouse.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-CDC42 Antibody (ZG-0101U) in WB
Positive WB detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse Brain whole cell lysate. All lanes: CDC42 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22, 22 kDa
Observed band size: 24 kDa
IHC
Figure 2 Rabbit Anti-CDC42 Antibody (ZG-0101U) in IHC
IHC image of ZG-0101U diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human CDC42
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDC42
- Species Reactivity
- Human, Mouse
- Clone
- 3C3
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Takenouchi-Kosaki Syndrome and Agenesis Of Corpus Callosum, Cardiac, Ocular, And Genital Syndrome.
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- TKS; G25K; CDC42Hs; G25K GTP-binding protein; GTP binding protein, 25kDa; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); growth-regulating protein; small GTP binding protein CDC42
- Gene ID
- 998
- UniProt ID
- P60953
Related Resources
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4073z | Mouse Anti-CDC42 Recombinant Antibody (clone 21B7) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MRO-0330-CN | Rabbit Anti-CDC42 Recombinant Antibody (clone CBACN-131) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-FY331 | Recombinant Rabbit Anti-CDC42 Antibody (clone R01-1C7) | WB | Rabbit IgG |
| VS-0923-FY36 | Recombinant Mouse Anti-CDC42 Antibody (VS-0923-FY36) | ELISA, WB | Mouse IgG1 |
| VS-0923-FY37 | Recombinant Mouse Anti-CDC42 Antibody (VS-0923-FY37) | ELISA, WB | Mouse IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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