CytoStream™ Rabbit Anti-CDC42 Recombinant Antibody (VS13-YC187) (CAT#: VS13-YC187)

• Immunogen
• Recombinant human CDC42, aa 30-191.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat CDC42

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF, FC

• Conjugate
• NONE

• Related Disease
• Takenouchi-Kosaki Syndrome and Temperature-Sensitive Lethal Mutation

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/1000
  Immunofluorescence (IF-cell): 1/500
  Flow cytometry (FC): 1/1000

• Alternative Names
• Cell Division Cycle 42; GTP Binding Protein, 25kDa; G25K GTP-Binding Protein; DJ224A6.1.1 (Cell Division Cycle 42 (GTP-Binding Protein, 25kD)); DJ224A6.1.2 (Cell Division Cycle 42 (GTP-Binding Protein, 25kD)); Cell Division Cycle 42 (GTP Binding Protein, 25kDa); Cell Division Cycle 42 (GTP-Binding Protein, 25kD);

• Gene ID
• 998

• UniProt ID
• P60953

• Long Name
• Cell Division Cycle 42

• Sequence Similarities
• Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.

• Cellular Localization
• Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane

• Protein Refseq
• NP_001034891.1; NP_001782.1; NP_426359.1

• Function
• Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed:15642749).
  Regulates cell migration (PubMed:17038317).
  In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed:14978216).
  Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed:33523862).
  Upon activation by CAMK2A, modulates dendritic spine structural plasticity by relaying CAMK2A transient activation to synapse-specific, long-term signaling (By similarity).
  Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed:26465210).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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