CytoStream™ Rabbit Anti-CDC42 Recombinant Antibody (VS13-YC187)
CAT#: VS13-YC187
The CytoStream™ Rabbit Anti-CDC42 Recombinant Antibody is specific for human, mouse and rat CDC42. CDC42 is crucial in developmental signaling pathways and diseases like Takenouchi-Kosaki syndrome, facilitating cellular interactions via small GTPases. The anti-CDC42 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4360/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69590/161451_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69590/161451_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69590/161451_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69590/161451_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69590/161451_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000070831-CDC42
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Specifications
- Immunogen
- Recombinant human CDC42, aa 30-191.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CDC42
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- Takenouchi-Kosaki Syndrome and Temperature-Sensitive Lethal Mutation
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Immunofluorescence (IF-cell): 1/500
Flow cytometry (FC): 1/1000
Target
- Alternative Names
- Cell Division Cycle 42; GTP Binding Protein, 25kDa; G25K GTP-Binding Protein; DJ224A6.1.1 (Cell Division Cycle 42 (GTP-Binding Protein, 25kD)); DJ224A6.1.2 (Cell Division Cycle 42 (GTP-Binding Protein, 25kD)); Cell Division Cycle 42 (GTP Binding Protein, 25kDa); Cell Division Cycle 42 (GTP-Binding Protein, 25kD);
- Gene ID
- 998
- UniProt ID
- P60953
- Long Name
- Cell Division Cycle 42
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
- Cellular Localization
- Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane
- Protein Refseq
- NP_001034891.1; NP_001782.1; NP_426359.1
- Function
- Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed:15642749).
Regulates cell migration (PubMed:17038317).
In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed:14978216).
Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed:33523862).
Upon activation by CAMK2A, modulates dendritic spine structural plasticity by relaying CAMK2A transient activation to synapse-specific, long-term signaling (By similarity).
Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed:26465210).
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CDC42"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4073z | Mouse Anti-CDC42 Recombinant Antibody (clone 21B7) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2473MZ | Recombinant Mouse Anti-Human CDC42 Antibody (clone 2) | ELISA, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0921MZ | Chicken Anti-Cdc42 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0330-CN | Rabbit Anti-CDC42 Recombinant Antibody (clone CBACN-131) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0101U | Rabbit Anti-CDC42 Recombinant Antibody (clone 3C3) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ1070 | Rabbit Anti-CDC42 Recombinant Antibody (VS3-CJ1070) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC659 | AbPlus™ Anti-CDC42 Magnetic Beads (VS-0724-YC659) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY387 | Anti-CDC42 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1265 | Anti-Human CDC42 Immunohistochemistry Kit | IHC |
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