Mouse Anti-CDH1 Recombinant Antibody (clone 9H2D3) (CAT#: ZG-0233U)

Figure 1 Mouse Anti-CDH1 Antibody (ZG-0233U) in WB

Positive WB detected in: Caco205 whole cell lysate All lanes: CDH1 antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 98, 91 kDa
Observed band size: 110, 120 kDa

Figure 2 Mouse Anti-CDH1 Antibody (ZG-0233U) in IF

Immunofluorescence staining of MCF-7 cells with ZG-0233U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 3 Mouse Anti-CDH1 Antibody (ZG-0233U) in FC

Overlay histogram showing MCF-7 cells stained with ZG-0233U (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.