Mouse Anti-CDH1 Recombinant Antibody (clone 9H2D3) (CAT#: ZG-0233U)
Mouse anti-CDH1 antibody (clone 9H2D3) displayed high specificity against the CDH1 protein of Human and Mouse.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Mouse Anti-CDH1 Antibody (ZG-0233U) in WB
Positive WB detected in: Caco205 whole cell lysate All lanes: CDH1 antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 98, 91 kDa
Observed band size: 110, 120 kDa
IF
Figure 2 Mouse Anti-CDH1 Antibody (ZG-0233U) in IF
Immunofluorescence staining of MCF-7 cells with ZG-0233U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 3 Mouse Anti-CDH1 Antibody (ZG-0233U) in FC
Overlay histogram showing MCF-7 cells stained with ZG-0233U (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant Human Cadherin-1 protein (155-707AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CDH1
- Species Reactivity
- Human, Mouse
- Clone
- 9H2D3
- Applications
- ELISA, WB, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Blepharocheilodontic Syndrome 1 and Gastric Cancer, Hereditary Diffuse
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:50-1:200
Target
- Alternative Names
- UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324; cadherin-1; CAM 120/80; E-cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; cell-CAM 120/80; epididymis secretory sperm binding protein; epithelial cadherin; uvomorulin
- Gene ID
- 999
- UniProt ID
- P12830
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1411CL | Anti-Human CDH1 Recombinant Antibody (hSC10.17) | Cyt, FuncS, Inhib | Humanized antibody |
| TAB-1411CL-S(P) | Anti-Human CDH1 Recombinant Antibody scFv Fragment (hSC10.17) | ELISA | Humanized antibody |
| TAB-1411CL-F(E) | Anti-Human CDH1 Recombinant Antibody Fab Fragment (hSC10.17) | ELISA | Humanized antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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