Mouse Anti-CDH1 Recombinant Antibody (clone 9H2D3)
CAT#: ZG-0233U
Mouse anti-CDH1 antibody (clone 9H2D3) displayed high specificity against the CDH1 protein of Human and Mouse.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CDH1 Antibody (ZG-0233U) in WB
Positive WB detected in: Caco205 whole cell lysate All lanes: CDH1 antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 98, 91 kDa
Observed band size: 110, 120 kDa
IF
Figure 2 Mouse Anti-CDH1 Antibody (ZG-0233U) in IF
Immunofluorescence staining of MCF-7 cells with ZG-0233U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 3 Mouse Anti-CDH1 Antibody (ZG-0233U) in FC
Overlay histogram showing MCF-7 cells stained with ZG-0233U (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28364/672_E7_2_selected.jpg
Normal Tissue
Figure 2 ParaThyroid gland
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4812/158154_A_3_8_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule Staining: Medium Intensity: Moderate Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_7_5.jpg
Normal Tissue
Figure 1 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72856/157200_A_5_5.jpg
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (BEWO, CACO-2, CAPAN-2, HaCaT, hTCEpi, MCF7, OE19, RT4, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000039068-CDH1
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Specifications
- Immunogen
- Recombinant Human Cadherin-1 protein (155-707AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CDH1
- Species Reactivity
- Human, Mouse
- Clone
- 9H2D3
- Applications
- ELISA, WB, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Blepharocheilodontic Syndrome 1 and Gastric Cancer, Hereditary Diffuse
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:50-1:200
Target
- Alternative Names
- UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324; cadherin-1; CAM 120/80; E-cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; cell-CAM 120/80; epididymis secretory sperm binding protein; epithelial cadherin; uvomorulin
- Gene ID
- 999
- UniProt ID
- P12830
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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