Mouse Anti-COL3A1 Recombinant Antibody (clone ABT-CIII) (CAT#: VS3-CJ284)
This product is a mouse antibody that recognizes COL3A1.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7583/1801_B1_14_cr59c25470e819f_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7583/1801_B1_14_cr59c25470e819f_selected.jpg
Figure 2 IHC staining of human nasopharynx
(Immunohistochemical staining of human nasopharynx shows distinct positivity in fibroblasts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7583/ihc_selected.jpg
(Immunohistochemical staining of human nasopharynx shows distinct positivity in fibroblasts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7583/ihc_selected.jpg
Figure 3 IHC staining of human bronchus
(Immunohistochemical staining of human bronchus shows distinct positivity in the submucosa.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16766/ihc_selected.jpg
(Immunohistochemical staining of human bronchus shows distinct positivity in the submucosa.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16766/ihc_selected.jpg
Figure 4 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic and membranous positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/ihc_selected.jpg
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic and membranous positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/ihc_selected.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_B_8_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_B_8_5.jpg
Figure 6 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_A_9_3.jpg
Figure 7 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_A_5_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_A_5_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (AF22, ASC diff, ASC TERT1, fHDF/TERT166, HHSteC, HSkMC)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168542-COL3A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168542-COL3A1
Specifications
- Immunogen
- Synthesized peptide derived from human Collagen Type III
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human COL3A1
- Species Reactivity
- Human
- Clone
- ABT-CIII
- Applications
- WB, IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500), Immunofluorescence (1:500-1:200).
Target
- Alternative Names
- Collagen alpha-1(III) chain; EDS4A; EDSVASC
- Gene ID
- 1281
- UniProt ID
- P02461
- Sequence Similarities
- Belongs to the fibrillar collagen family.
- Cellular Localization
- Extracellular matrix, Secreted
- Post Translation Modifications
- Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
- Protein Refseq
- NP_000081.1
- Function
- Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0910-FY-F(E) | Mouse Anti-COL3A1 Recombinant Antibody (clone 4E5F4A11); scFv Fragment | ELISA | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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