Human Anti-CSF1 Recombinant Antibody (HPAB-0150CQ)
CAT#: HPAB-0150CQ
This product is a recombinant Human antibody that recognizes CSF1. The antibody was purified by affinity chromatography.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1709_G12_12_cr5800dfbe06bb5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1876_A10_31_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1769_A4_33_blue_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (ASC diff, BJ hTERT+, GAMG, HHSteC, HSkMC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184371-CSF1
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Specifications
- Immunogen
- Human M-CSF
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human CSF1
- Species Reactivity
- Human
- Applications
- ELISA, WB, FC, Inhib, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Versatile Antibody for Flow Cytometry
This product has proven to be very versatile in our lab, particularly for Flow Cytometry. It binds specifically to human CSF1, and the purity of the recombinant product ensures we get reproducible data every time. It has become a standard reagent for our cytokine profiling projects.
Successful Inhibition in Cell Culture
We utilized this recombinant human antibody for inhibition studies involving Colony Stimulating Factor 1. The antibody effectively neutralized the biological activity of CSF1 in our cell-based assays, allowing us to investigate the downstream signaling pathways of the CSF1 receptor with great precision.
Q&As
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Does this antibody neutralize the biological activity of CSF1?
A: Yes, this Recombinant Human Anti-CSF1 Antibody is validated for Inhibition (Inhib) and Functional Studies (FuncS). It binds to Human Macrophage Colony-Stimulating Factor (M-CSF) and can be used to study the biological effects of this cytokine, such as its interaction with the CSF1 receptor in cell assays.
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What level of purity can I expect from this recombinant antibody?
A: This is a recombinant Human antibody expressed in Human cells. It is purified by affinity chromatography, ensuring a purity of greater than 95% as determined by SDS-PAGE analysis. This high level of purity guarantees high reliability and reproducibility for your sensitive research experiments.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| MOR-0824 | Hi-Affi™ Rabbit Anti-CSF1 Recombinant Antibody (clone DS824AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0532-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0532-CN) | ELISA, FC, Neut | Mouse IgG |
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| HPAB-0534-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0534-CN) | ELISA, FC, Neut | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0532-CN-S(P) | Mouse Anti-CSF1 Recombinant Antibody; scFv Fragment (HPAB-0532-CN-S(P)) | ELISA, FC, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0533-CN-S(P) | Mouse Anti-CSF1 Recombinant Antibody; scFv Fragment (HPAB-0533-CN-S(P)) | ELISA, FC, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0534-CN-S(P) | Mouse Anti-CSF1 Recombinant Antibody; scFv Fragment (HPAB-0534-CN-S(P)) | ELISA, FC, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0532-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0532-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0533-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0533-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0534-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0534-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-029ML | Afuco™ Anti-CSF1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-029ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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