Mouse Anti-CTNNB1 Recombinant Antibody (clone 4F2)

Name: Mouse Anti-CTNNB1 Recombinant Antibody (clone 4F2)
SKU: ZG-0073J
Rating: 5 (1 reviews)

CAT#: ZG-0073J

This product is a mouse antibody that recognizes CTNNB1.

Tested Data Gene Expression

Figure 1 Mouse Anti-CTNNB1 Recombinant Antibody (clone 4F2) in IF

Immunofluorescence analysis of Human-stomach-cancer tissue. 1, Catenin-βMonoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

Figure 2 Mouse Anti-CTNNB1 Recombinant Antibody (clone 4F2) in IF

Immunofluorescence analysis of Mouse-spleen tissue. 1.Catenin-β Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

Figure 3 Mouse Anti-CTNNB1 Recombinant Antibody (clone 4F2) in WB

Western blot analysis of 1) Hela, 2) 293T, 3) Mouse Liver Tissue, 4) Rat Liver Tissue using Catenin-β Monoclonal Antibody.

IHC

Figure 4 Mouse Anti-CTNNB1 Recombinant Antibody (clone 4F2) in IHC

Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Catenin-β Monoclonal Antibody.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/if_selected.jpg

Normal Tissue Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human colon

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/108/ihc_selected.jpg

Normal Tissue Figure 4 IHC staining of human liver

Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1950/ihc_selected.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_B_8_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_4.jpg

Normal Tissue Figure 8 Pancreas

Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_1_3.jpg

Normal Tissue Figure 9 Kidney

Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_5.jpg

Normal Tissue Figure 10 Testis

Sertoli cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_4_6.jpg

RNA Expression Figure 11 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168036-CTNNB1

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Specifications

Immunogen
Recombinant Protein of Catenin-β

Host Species
Mouse

Type
Mouse IgG

Specificity
Human CTNNB1

Species Reactivity
Human, Mouse, Rat

Clone
4F2

Applications
WB, IF, ICC, IHC-P

Product Property

Storage
Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Western Blot, Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Paraffin.

Target

Alternative Names
EVR7; CTNNB; MRD19; NEDSDV; armadillo; catenin beta-1; catenin (cadherin-associated protein), beta 1, 88kDa

Gene ID
1499

UniProt ID
P35222

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Related Diseases

Cushing Syndrome

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0073J.pdf

MSDS

MSDS - ZG-0073J.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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