Rabbit Anti-CTSB Recombinant Antibody (VS13-YC270)
CAT#: VS13-YC270
The Rabbit Anti-CTSB Recombinant Antibody is specific for human CTSB. CTSB, cathepsin B, contributes to diverse pathways like immune response, impacting conditions such as cystic fibrosis and peptidase activities. The anti-CTSB antibody can be used for Western blot (1/500-1/1000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18156/150_G3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/457/613_B10_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells. Brunner's glands were also positive while Paneth cells were negative.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18156/41696_A_8_2_selected.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48998/167916_A_9_4.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48998/167916_B_9_5.jpg
Normal Tissue
Figure 6 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48998/167916_B_1_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48998/167916_A_7_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+, fHDF/TERT166)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164733-CTSB
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Specifications
- Immunogen
- Recombinant human Cathepsin B, aa 7-176.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CTSB
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
- Related Disease
- Keratolytic Winter Erythema and Occlusion Of Gallbladder
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CTSB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1862z | Mouse Anti-CTSB Recombinant Antibody (clone 41C6) | WB, ICC, IF, IHC | Mouse IgG1 |
| MOB-0334CT | Recombinant Mouse anti-Human CTSB Monoclonal antibody (DC242) | IHC-P | |
| MRO-1724-CN | Rabbit Anti-CTSB Polyclonal Antibody (MRO-1724-CN) | WB, IF | Rabbit IgG |
| VS3-WK1393 | Rabbit Anti-CTSB Recombinant Antibody (VS3-WK1393) | WB, ELISA | Rabbit IgG |
| VS3-FY426 | Recombinant Rabbit Anti-Ctsb Antibody (clone R08-7H4) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0866 | Hi-Affi™ Rabbit Anti-CTSB Recombinant Antibody (clone DS866AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-015CN | Mouse Anti-CTSB Recombinant Antibody (clone 2A2) | ELISA, WB | Mouse IgG |
| NS-015CN-F(E) | Mouse Anti-CTSB Recombinant Antibody (clone 2A2); Fab Fragment | ELISA, WB | Mouse Fab |
| NS-015CN-S(P) | Mouse Anti-CTSB Recombinant Antibody (clone 2A2); scFv Fragment | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC144 | AbPlus™ Anti-CTSB Magnetic Beads (VS-0724-YC144) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1781 | Anti-Mouse CTSB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC78 | Recombinant Anti-CTSB Eliminating Antibody, pH-Sensitive (2A2 var.) | Antigen-Sweeping In Vivo. | Mouse IgG |
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