Anti-DDR1 Antibody Prodrug, Protease Activated (APE05570)
CAT#: VS-1025-YC178
The Anti-DDR1 Antibody Prodrug, Protease Activated (APE05570) is an antibody prodrug comprised of a recombinant human antibody targeting human DDR1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to DDR1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to DDR1 effectively. DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase activated by binding to collagen in the extracellular matrix, which promotes cell survival and invasion. Its overexpression is associated with poor prognosis in a variety of tumors, particularly ovarian and breast cancer.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57194/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25656/57796_A_9_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25656/57796_B_7_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25656/57796_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25656/57796_A_5_6.jpg
Normal Tissue
Figure 6 Skin
Melanocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25656/57796_B_7_1.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HaCaT, HBEC3-KT, RT4, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204580-DDR1
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human DDR1
- Target
- DDR1
- Species Reactivity
- Human, Mouse
- Isotype
- IgG
- Clone
- APE05570
- Affinity Constant
- KD = 3.5 nM
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
- Alternative Names
- Discoidin Domain Receptor Tyrosine Kinase 1; CD167 Antigen-Like Family Member A; EC 2.7.10.1; EDDR1; NTRK4; PTK3A; HGK2; RTK6; TRKE; CAK; NEP
- Gene ID
- 780
- UniProt ID
- Q08345
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Datasheet
MSDS
COA
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See other products for "Clone APE05570"
- CAT
- Product Name
See other products for "DDR1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-436 | Mouse Anti-DDR1 Recombinant Antibody (clone mAb 3E3) | ELISA, FC | Mouse IgG |
| MOB-1224CT | Recombinant Mouse anti-Human DDR1 Monoclonal antibody (3H5F23) | ELISA, IHC-P, WB | |
| HPAB-0077-CN | Human Anti-DDR1 Recombinant Antibody (clone APE05570) | ELISA, FC | Human IgG |
| HPAB-0078-CN | Human Anti-DDR1 Recombinant Antibody (clone APE05571) | ELISA, FC | Human IgG |
| HPAB-0061-LSX | Mouse Anti-DDR1 Recombinant Antibody (clone AB2026) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-433 | Mouse Anti-DDR1 Recombinant Antibody (clone 3E3); Fab Fragment | ELISA, FC | Mouse Fab |
| HPAB-0077-CN-F(E) | Human Anti-DDR1 Recombinant Antibody (clone APE05570); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0078-CN-F(E) | Human Anti-DDR1 Recombinant Antibody (clone APE05571); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0061-LSX-F(E) | Mouse Anti-DDR1 Recombinant Antibody (clone AB2026); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0062-LSX-F(E) | Mouse Anti-DDR1 Recombinant Antibody (clone AB2039); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-433 | Mouse Anti-DDR1 Recombinant Antibody (clone mAb 3E3); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-0077-CN-S(P) | Human Anti-DDR1 Recombinant Antibody (clone APE05570); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0078-CN-S(P) | Human Anti-DDR1 Recombinant Antibody (clone APE05571); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0061-LSX-S(P) | Mouse Anti-DDR1 Recombinant Antibody (clone AB2026); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0062-LSX-S(P) | Mouse Anti-DDR1 Recombinant Antibody (clone AB2039); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-176MZ | Anti-Human DDR1 Recombinant Antibody (DDR1-ab1) | FuncS | Human antibody |
| TAB-177MZ | Anti-Human DDR1 Recombinant Antibody (DDR1-ab2) | FuncS | Human antibody |
| TAB-178MZ | Anti-Human DDR1 Recombinant Antibody (DDR1-ab3) | FuncS | Human antibody |
| TAB-176MZ-S(P) | Anti-Human DDR1 Recombinant Antibody scFv Fragment (DDR1-ab1) | IF | Human antibody |
| TAB-177MZ-S(P) | Anti-Human DDR1 Recombinant Antibody scFv Fragment (DDR1-ab2) | IF | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-179MZ | Anti-Human DDR1 Recombinant Antibody (AB2004) | ELISA | |
| TAB-180MZ | Anti-Human DDR1 Recombinant Antibody (AB2009) | ELISA | |
| TAB-181MZ | Anti-Human DDR1 Recombinant Antibody (AB2041) | ELISA | |
| TAB-182MZ | Mouse Anti-DDR1 Recombinant Antibody (TAB-182MZ) | FC | Mouse IgG |
| TAB-183MZ | Anti-Human DDR1 Recombinant Antibody (1F10) | WB, ELISA, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0953 | Hi-Affi™ Rabbit Anti-DDR1 Recombinant Antibody (clone DS953AB) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1235 | AbPlus™ Anti-DDR1 Magnetic Beads (VS-0724-YC1235) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY638 | Anti-DDR1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC431 | Recombinant Anti-DDR1 Vesicular Antibody, EV Displayed (VS-0425-YC431) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC97 | SmartAb™ Recombinant Anti-DDR1 pH-dependent Antibody (Clone AB2004) | ELISA | Mouse IgG |
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