Human Anti-DLL3 Recombinant Antibody (clone 7B7)
CAT#: ZG-0136U
Human anti-DLL3 antibody (clone 7B7) displayed high specificity against the DLL3 protein of Human.
Tested Data
Gene Expression
FC
Figure 1 Human Anti-DLL3 Antibody (ZG-0136U) in FC
Untransfected HEK293 cells (green line) and transfected Human DLL3 HEK293 stable cells (red line) were stained with anti-DLL3 antibody (rovalpituzumab-like) (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG1 Fc antibody and analyzed with flow cytometry.
ELISA
Figure 2 Human Anti-DLL3 Antibody (ZG-0136U) in ELISA
The Binding Activity of DLL3 with Anti-DLL3 Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 at 2 μg/mL can bind Anti-DLL3 Recombinant Antibody (ZG-0136U), the EC50 is 1.102-1.707 ng/mL.
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Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24559/1931_D10_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56533/1219_G4_3_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090932-DLL3
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Specifications
- Immunogen
- Recombinant Human DLL3 protein
- Host Species
- Human
- Type
- Humanized IgG1
- Specificity
- Human DLL3
- Species Reactivity
- Human
- Clone
- 7B7
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
- Related Disease
- Spondylocostal Dysostosis 1, Autosomal Recessive and Spondylocostal Dysostosis, Autosomal Recessive
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry. Specific conditions should be optimized by the end user.
FC:1:50-1:200
Target
REVIEWS AND Q&AS
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Customer Review
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Easy-to-use Packaging
The packing was manageable and practical. It greatly simplified accessing and storing the product.
Correct Record-Keeping
The product manual was precise and guided us in making the best use of it. Very educational datasheet.
High Recuperation Rates
In our experiments, we saw significant recovery rates. Extremely happy with the antibody's functionality.
Q&As
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How does antibody valency influence binding?
A: Antibody bivalency improves binding affinity and stability when interacting with multivalent antigens.
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Could antibodies be used to detect protein-protein interactions?
A: Co-immunoprecipitation using particular antibodies is a useful method for studying protein-protein interactions.
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What effect do stabilizers have on antibody solutions?
A: Stabilizers, such BSA or glycerol, keep antibodies active and prevent degradation over time.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| HPAB-2100LY-F(E) | Mouse Anti-DLL3 Recombinant Antibody; Fab Fragment (HPAB-2100LY-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-2101LY-F(E) | Mouse Anti-DLL3 Recombinant Antibody; Fab Fragment (HPAB-2101LY-F(E)) | ELISA, FC | Mouse Fab |
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| HPAB-2104LY-F(E) | Mouse Anti-DLL3 Recombinant Antibody; Fab Fragment (HPAB-2104LY-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
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| HPAB-2112LY | Mouse Anti-DLL3 Recombinant Antibody (HPAB-2112LY) | ELISA, FC | Mouse IgG |
| HPAB-2113LY | Mouse Anti-DLL3 Recombinant Antibody (HPAB-2113LY) | ELISA, FC | Mouse IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2112LY-S(P) | Mouse Anti-DLL3 Recombinant Antibody; scFv Fragment (HPAB-2112LY-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-2113LY-S(P) | Mouse Anti-DLL3 Recombinant Antibody; scFv Fragment (HPAB-2113LY-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-2114LY-S(P) | Mouse Anti-DLL3 Recombinant Antibody; scFv Fragment (HPAB-2114LY-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-2115LY-S(P) | Mouse Anti-DLL3 Recombinant Antibody; scFv Fragment (HPAB-2115LY-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-2116LY-S(P) | Mouse Anti-DLL3 Recombinant Antibody; scFv Fragment (HPAB-2116LY-S(P)) | ELISA, FC | Mouse scFv |
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