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  • Mouse Anti-E2F1 Recombinant Antibody (VS3-FY2693)

Mouse Anti-E2F1 Recombinant Antibody (VS3-FY2693) (CAT#: VS3-FY2693)

The anti-E2F1 antibody is specifically engineered to target human E2F1 protein. It shows capability of cross-reacting with mouse and rat. This monoclonal antibody proves valuable in disease research related to Retinoblastoma and Squamous Cell Carcinoma.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Formats:
  • Isotype Switching:
  • Fc Engineering:
  • Modalities:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Figure 3 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
Figure 4 IHC staining of human colon
Figure 4 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
Figure 7 Liver
Figure 7 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
Figure 9 Testis
Figure 9 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
Figure 10 Bone marrow
Figure 10 Bone marrow
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1

Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • E2F transcription factor 1 of human, mouse and rat origins
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, ICC, IP
  • Conjugate
  • Unconjugated, FITC
  • Related Disease
  • Retinoblastoma and Squamous Cell Carcinoma

Product Property

  • Clonality
  • Monoclonal Antibody
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Immunoprecipitation: 1/20
    Western Blot: 1/500-1/1000
    Immunocytochemistry: 1/50-1/200

Target

  • Alternative Names
  • E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
  • Sequence Similarities
  • Belongs to the E2F/DP family.
  • Cellular Localization
  • Nucleus
  • Post Translation Modifications
  • Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase
    Ubiquitinated via 'Lys-63'-linked ubiquitin, leading to its degradation
    Acetylation stimulates DNA-binding.
    Methylation at Lys-185 by SETD7 promotes E2F1 ubiquitin-dependent proteasomal degradation.
    Modification sites at PhosphoSitePlus
    Modification sites at neXtProt
  • Function
  • The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed:10675335, 12717439, 17704056).
    E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed:10675335, 12717439, 17704056).
    It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed:8170954).
    Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters.

Recommended Products

Secondary Antibody

Isotype Control

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "E2F1"

Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products Rabbit Monoclonal Antibody Products
CAT Product Name Application Type
MOB-4001z Mouse Anti-E2F1 Recombinant Antibody (clone 30B9) WB, IP, IF, ELISA Mouse IgG2b, κ
MOB-0156CT Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) ICC/IF, IP, WB
ZG-0266J Mouse Anti-E2F1 Recombinant Antibody (ZG-0266J) WB, ICC Mouse IgG
ZG-0641J Rabbit Anti-E2F1 Recombinant Antibody (clone 1D12) ELISA, WB, IHC, IF, FC Rabbit IgG
VS3-WK839 Rabbit Anti-E2F1 Recombinant Antibody (VS3-WK839) WB, ICC, IF, IHC, IP, FC Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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