Rabbit Anti-EIF4A1 Recombinant Antibody (clone 7C3) (CAT#: ZG-0053U)
The purified Rabbit Anti-EIF4A1 Recombinant Antibody (clone 7C3) displayed high specificity against the EIF4A1 protein of human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-EIF4A1 Antibody (ZG-0053U) in WB
Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate. All lanes: eIF4A1 Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 47 kDa
Observed band size: 50 kDa
IF
Figure 2 Rabbit Anti-EIF4A1 Antibody (ZG-0053U) in IF
Immunofluorescence staining of Hela Cells with ZG-0053U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human eIF4A1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EIF4A1
- Species Reactivity
- Human
- Clone
- 7C3
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Ovary Sarcoma and Parkinson Disease, Late-Onset
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; EC 3.6.4.13; ATP-dependent RNA helicase eIF4A-1; EIF4A1; DDX2A EIF4A
- Gene ID
- 1973
- UniProt ID
- P60842
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "EIF4A1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1125 | Hi-Affi™ Rabbit Anti-EIF4A1 Recombinant Antibody (clone DS1125AB) | WB, ICC, IF, FC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0012C | Mouse Anti-EIF4A1 Recombinant Antibody (clone M8) | WB, IHC-P, IF/ICC | Mouse IgG |
| VS3-CJ1067 | Mouse Anti-EIF4A1 Recombinant Antibody (VS3-CJ1067) | WB, ICC, IF, IHC | Mouse IgG |
| VS3-FY485 | Recombinant Rabbit Anti-EIF4A1 Antibody (clone R04-2K6) | WB, IP | Rabbit IgG |
| VS4-WK157 | Mouse Anti-EIF4A1 Recombinant Antibody (VS4-WK157) | ELISA, WB, IHC, IF | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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