Mouse Anti-EIF4A1 Recombinant Antibody (clone M8) (CAT#: ZG-0012C)

This product is a mouse antibody that recognizes EIF4A1. It can be used in a wide variety of applications, such as: WB, IHC-P, IF/ICC.


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  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IHC

Figure 1 Mouse Anti-EIF4A1 Antibody (clone M8) in IHC

Figure 1 Mouse Anti-EIF4A1 Antibody (clone M8) in IHC

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue. 1, eIF4A1 Monoclonal Antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IHC

Figure 2 Mouse Anti-EIF4A1 Antibody (clone M8) in IHC

Figure 2 Mouse Anti-EIF4A1 Antibody (clone M8) in IHC

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.1, eIF4A1 Monoclonal Antibody (M8) was diluted at 1:200 (4°C, overnight).2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).Negative control was used by Secondary antibody only.

IHC

Figure 3 Mouse Anti-EIF4A1 Antibody (clone M8) in IHC

Figure 3 Mouse Anti-EIF4A1 Antibody (clone M8) in IHC

Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue.1, eIF4A1 Monoclonal Antibody (M8) was diluted at 1:200 (4°C, overnight).2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).Negative control was used by Secondary antibody only.

IF

Figure 4 Mouse Anti-EIF4A1 Antibody (clone M8) in IF

Figure 4 Mouse Anti-EIF4A1 Antibody (clone M8) in IF

Immunofluorescence analysis of Mouse-spleen tissue. 1, eIF4A1Monoclonal Antibody (M8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

IF

Figure 5 Mouse Anti-EIF4A1 Antibody (clone M8) in IF

Figure 5 Mouse Anti-EIF4A1 Antibody (clone M8) in IF

IF analysis of Hela with Antibody (Left) and DAPI (Right) diluted at 1:100.

WB

Figure 6 Mouse Anti-EIF4A1 Antibody (clone M8) in WB

Figure 6 Mouse Anti-EIF4A1 Antibody (clone M8) in WB

WB analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse Brain tissue.


Specifications

  • Immunogen
  • Synthetic Peptide of eIF4A1
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • eIF4A1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • M8
  • Applications
  • WB, IHC-P, IF/ICC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Storage
  • Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.

Target

  • Alternative Names
  • Eukaryotic Translation Initiation Factor 4A1; ATP-Dependent RNA Helicase EIF4A-1; EIF-4A-I; EIF4A-I; EIF4A; DDX2A; Eukaryotic Translation Initiation Factor 4A, Isoform 1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "EIF4A1"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1125 Hi-Affi™ Rabbit Anti-EIF4A1 Recombinant Antibody (clone DS1125AB) WB, ICC, IF, FC Rabbit IgG

Recombinant Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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