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    Mouse Anti-EIF4A1 Recombinant Antibody (VS3-CJ1067) (CAT#: VS3-CJ1067)

    This product is a mouse antibody that recognizes human, mouse, and rat endogenous levels of eIF4AI protein.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/68286/1754_D9_13_cr57f3db32859db_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human placenta
    Figure 2 IHC staining of human placenta
    (Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/ihc_selected.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_7_3.jpg
    Figure 4 Testis
    Figure 4 Testis
    (Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_4_6.jpg
    Figure 5 Lymph node
    Figure 5 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_8_8.jpg
    RNA Expression
    Figure 6 RNA cell line category: Low cell line specificity
    Figure 6 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161960-EIF4A1

    Specifications

    • Immunogen
    • KLH-conjugated synthetic peptide encompassing a sequence of human eIF4AI
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human, Mouse, Rat endogenous levels of eIF4AI protein
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • WB, ICC, IF, IHC
    • Conjugate
    • Unconjugated

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
    • Preservative
    • 0.01% Sodium Azide
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Shipped at 4°C

    Applications

    • Application Notes
    • This antibody has been tested for use in Western Blot (1:1000-1:3000), Immunocytochemistry/Immunofluorescence (1:100-1:200), Immunohistochemistry (1:50-1:200).

    Target

    • Alternative Names
    • DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
    • Sequence Similarities
    • Belongs to the DEAD box helicase family. eIF4A subfamily.
    • Cellular Localization
    • Cytoplasm, Cytosol
    • Function
    • ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "EIF4A1"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Rabbit Monoclonal Antibody Products IgG Antibody Products Non-Human Primate (NHP) Antibody Products
    CAT Product Name Application Type
    MOR-1125 Hi-Affi™ Rabbit Anti-EIF4A1 Recombinant Antibody (clone DS1125AB) WB, ICC, IF, FC Rabbit IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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