Mouse Anti-EIF4A1 Recombinant Antibody (VS3-CJ1067)
CAT#: VS3-CJ1067
This product is a mouse antibody that recognizes human, mouse, and rat endogenous levels of eIF4AI protein.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68286/1754_D9_13_cr57f3db32859db_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161960-EIF4A1
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence of human eIF4AI
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat endogenous levels of eIF4AI protein
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:3000), Immunocytochemistry/Immunofluorescence (1:100-1:200), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
- Gene ID
- 1973
- UniProt ID
- P60842
- Sequence Similarities
- Belongs to the DEAD box helicase family. eIF4A subfamily.
- Cellular Localization
- Cytoplasm, Cytosol
- Protein Refseq
- NP_001191439.1; NP_001407.1
- Function
- ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "EIF4A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1125 | Hi-Affi™ Rabbit Anti-EIF4A1 Recombinant Antibody (clone DS1125AB) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0012C | Mouse Anti-EIF4A1 Recombinant Antibody (clone M8) | WB, IHC-P, IF, ICC | Mouse IgG |
| ZG-0053U | Rabbit Anti-EIF4A1 Recombinant Antibody (clone 7C3) | ELISA, WB, IF | Rabbit IgG |
| VS3-FY485 | Recombinant Rabbit Anti-EIF4A1 Antibody (clone R04-2K6) | WB, IP | Rabbit IgG |
| VS4-WK157 | Mouse Anti-EIF4A1 Recombinant Antibody (VS4-WK157) | ELISA, WB, IHC, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY182 | Rabbit Anti-NHP EIF4A1 Recombinant Antibody (VS-1024-XY182) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2223 | Anti-EIF4A1 Immunohistochemistry Kit | IHC |
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