Mouse Anti-EIF4A1 Recombinant Antibody (VS3-CJ1067) (CAT#: VS3-CJ1067)
This product is a mouse antibody that recognizes human, mouse, and rat endogenous levels of eIF4AI protein.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68286/1754_D9_13_cr57f3db32859db_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68286/1754_D9_13_cr57f3db32859db_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/ihc_selected.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_7_3.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_4_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11689/32631_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161960-EIF4A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161960-EIF4A1
Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence of human eIF4AI
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat endogenous levels of eIF4AI protein
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:3000), Immunocytochemistry/Immunofluorescence (1:100-1:200), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
- Gene ID
- 1973
- UniProt ID
- P60842
- Sequence Similarities
- Belongs to the DEAD box helicase family. eIF4A subfamily.
- Cellular Localization
- Cytoplasm, Cytosol
- Protein Refseq
- NP_001191439.1; NP_001407.1
- Function
- ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "EIF4A1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1125 | Hi-Affi™ Rabbit Anti-EIF4A1 Recombinant Antibody (clone DS1125AB) | WB, ICC, IF, FC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0012C | Mouse Anti-EIF4A1 Recombinant Antibody (clone M8) | WB, IHC-P, IF/ICC | Mouse IgG |
| ZG-0053U | Rabbit Anti-EIF4A1 Recombinant Antibody (clone 7C3) | ELISA, WB, IF | Rabbit IgG |
| VS3-FY485 | Recombinant Rabbit Anti-EIF4A1 Antibody (clone R04-2K6) | WB, IP | Rabbit IgG |
| VS4-WK157 | Mouse Anti-EIF4A1 Recombinant Antibody (VS4-WK157) | ELISA, WB, IHC, IF | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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