Mouse Anti-ENO1 Recombinant Antibody (clone 4D11F5) (CAT#: ZG-0251U)

Figure 1 Mouse Anti-ENO1 Antibody (ZG-0251U) in WB

Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate. All lanes ENO1 antibody at 1: 10000
Secondary
Goat polyclonal to mouse IgG at 1/10000 dilution
Predicted band size: 47 KDa
Observed band size: 47 KDa
Exposure time: 10s

Figure 2 Mouse Anti-ENO1 Antibody (ZG-0251U) in IF

Immunofluorescence staining of Hela cells with ZG-0251U at 1:270, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 3 Mouse Anti-ENO1 Antibody (ZG-0251U) in FC

Overlay histogram showing MCF-7 cells stained with ZG-0251U (red line) at 1:550. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Figure 4 Mouse Anti-ENO1 Antibody (ZG-0251U) in IP

Immunoprecipitating ENO1 in HepG2 whole cell lysate Lane 1: Mouse control IgG (1µg) in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0251U (1µl) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)