Human Anti-EPHA2 Antibody (3F2), CHO Stable Cell Pool

Name: Human Anti-EPHA2 Antibody (3F2), CHO Stable Cell Pool
SKU: VS-1025-QX41
Rating: 5 (1 reviews)

CAT#: VS-1025-QX41

This CHO Stable Cell Pool represents a genetically engineered Chinese Hamster Ovary (CHO) cell population specifically designed for the robust and consistent expression of recombinant 3F2. 3F2 is a Human monoclonal antibody that targets EPHA2. This cell pool was generated through the stable integration of genes encoding the heavy and light chains of 3F2 into the CHO genome, followed by selection and enrichment for high-expressing cells. This cell pool provides rapid production of research-grade antibodies for non-GLP preclinical studies.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A549

Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles, plasma membrane, the Golgi apparatus & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10464/991_D3_3_selected.jpg

RNA Expression Figure 2 RNA cell line category: Cell line enhanced (CAPAN-2, PC-3, U-251 MG, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142627-EPHA2

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Specifications

Host Cell
CHO

Product Type
Stable Cell Pool

Cell Viability
>90%

Mycoplasma Detection
Negative

Bacteria Detection
Negative

Growth Properties
Suspension

Culture Medium
VersaCHO™ Medium (VS-1025-QX582)

Complete Growth Medium
VersaCHO™ Medium (VS-1025-QX582), VersaCHO™ Feed Medium (VS-1025-QX583), VersaCHO™ Feed Supplement (VS-1025-QX584)

Freeze Medium
90% VersaCHO™ Medium, 10% DMSO

Growth Conditions
37°C, 5% CO₂

Size
Each vial contains at least 1×10^6 cells in 1 mL

Shipping
Dry ice

Storage
Store in liquid nitrogen immediately upon receipt. Avoid multiple freeze/thaw cycles. Utilize cells within one year of the delivery date.

Antibody Overview

Host Species
Human

Antibody Type
Human Fab

Antibody Tag
His tag

Clone
3F2

Target
EPHA2

Species Reactivity
Human

Target

Alternative Names
EPH Receptor A2; Tyrosine-Protein Kinase Receptor ECK; EC 2.7.10.1; ECK; Epithelial Cell Receptor Protein Tyrosine Kinase; Ephrin Type-A Receptor 2; Soluble EPHA2 Variant 1; Epithelial Cell Kinase;

Gene ID
1969

UniProt ID
P29317

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Downloadable Resources

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Datasheet

Datasheet - VS-1025-QX41.pdf

MSDS

MSDS - VS-1025-QX41.pdf

COA

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