Human Anti-EPHA2 Recombinant Antibody (clone 1C1) (CAT#: PABL-083)
Recombinant Human Antibody (1C1) is capable of binding to EphA2, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-EphA2 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-EphA2 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Figure 1 Internalization of human EphA2 following incubation with anti-human EphA2 antibodies. mAb and Fab 1C1 efficiently internalize human EphA2, whereas mAbs 3F2 and 1C9 induce only weak to no internalization at 37 °C. No internalization of the antibodies was observed at 4 °C.
Peng, L., Oganesyan, V., Damschroder, M. M., Wu, H., & Dall'Acqua, W. F. (2011). Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody. Journal of molecular biology, 413(2), 390-405.
Figure 2 Identification of non-competing anti-EphA2 mAbs.
(A) Histograms for EphA2-positive cell lines HEC-1-A and PC-3 first stained with unlabeled EphA2 mAb 1C1 or 3035, an IgG control, or buffer only, followed by staining with either labeled 1C1 (1C1-A488) or 3035 (3035-A488). A488 fluorescence is detected by conducting flow cytometry on 10,000 live, single cells.
Liao-Chan, S., Daine-Matsuoka, B., Heald, N., Wong, T., Lin, T., Cai, A. G.,... & Theunissen, J. W. (2015). Quantitative assessment of antibody internalization with novel monoclonal antibodies against alexa fluorophores. PloS one, 10(4), e0124708.
Figure 3 Fluorescence-activated cell-sorting analysis of binding of 1C1 to human EphA2 and EphA4 variants transiently expressed on the surface of HEK 293F cells. Expression of those variants was monitored using a cocktail of antibodies recognizing both human EphA2 and EphA4. MFI, mean fluorescence intensity; SSC, side scatter characteristics.
Peng, L., Oganesyan, V., Damschroder, M. M., Wu, H., & Dall'Acqua, W. F. (2011). Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody. Journal of molecular biology, 413(2), 390-405.
Figure 4 Direct binding of 1C1 to sEphA2/ECD, sEphA2/LBD, and sEphA4/LBD-EphA2 variants by ELISA. EphA2 LBD contains the 1C1 epitope. Standard deviations are indicated by error bars and represent triplicate measurements within the same experiment.
Peng, L., Oganesyan, V., Damschroder, M. M., Wu, H., & Dall'Acqua, W. F. (2011). Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody. Journal of molecular biology, 413(2), 390-405.
Figure 5 Induction of EphA2 phosphorylation (a) and degradation (b) by anti-human EphA2 antibodies. mAb and Fab 1C1 trigger efficient phosphorylation and degradation of human EphA2, whereas mAbs 3F2 and 1C9 exhibit only weak to no activity.
Peng, L., Oganesyan, V., Damschroder, M. M., Wu, H., & Dall'Acqua, W. F. (2011). Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody. Journal of molecular biology, 413(2), 390-405.
Figure 6 Competition binding of anti-human EphA2 antibodies to sEphA2/ECD. 1C1 binds to an epitope distinct from that of 1C9 and 3F2.
Peng, L., Oganesyan, V., Damschroder, M. M., Wu, H., & Dall'Acqua, W. F. (2011). Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody. Journal of molecular biology, 413(2), 390-405.
Specifications
- Immunogen
- Human EPH receptor A2
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human EPHA2
- Species Reactivity
- Human
- Clone
- 1C1
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence, Flow Cytometry and Enzyme-linked Immunosorbent Assay. For details, refer to published data.
Target
- Alternative Names
- EPHA2; EPH receptor A2; ECK; CTPA; ARCC2; CTPP1; CTRCT6; ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase
- Gene ID
- 1969
- UniProt ID
- A0A024QZA8
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-381MZ | Anti-Human EPHA2 Recombinant Antibody (LUCA40) | FC | |
| TAB-382MZ | Anti-Human EPHA2 Recombinant Antibody (SPL1) | IHC, WB, sELISA | |
| TAB-383MZ | Anti-Human EPHA2 Recombinant Antibody (SG5 ) | IHC, WB, sELISA | |
| TAB-384MZ | Mouse Anti-EPHA2 Recombinant Antibody (TAB-384MZ) | ELISA | Mouse IgG |
| TAB-380MZ-S(P) | Mouse Anti-EPHA2 Recombinant Antibody; scFv Fragment (TAB-380MZ-S(P)) | IHC, WB, sELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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