Recombinant Human Anti-EPOR Antibody (Ab12) (CAT#: HPAB-1692-FY)
This product is a recombinant antibody that recognizes EPOR. The monoclonal antibody Ab12 reacts with the epitope of EPOR.
Specific Inquiry
Activ
Figure 1 EPO-dependent cell proliferation activity of Ab12 variants correlates inversely with Kd.
Ab variants with faster off-rates (Abs 12.6, 12.70, and 12.56) stimulate the proliferation of EPO-responsive F36E human erythroleukemic cells more effectively than does Ab12.
Lacy, S. E., DeVries, P. J., Xie, N., Fung, E., Lesniewski, R. R., & Reilly, E. B. (2008). The potency of erythropoietin-mimic antibodies correlates inversely with affinity. The Journal of Immunology, 181(2), 1282-1287.
Activ
Figure 2 CFU-E colony forming activity of Ab12 variants correlates inversely with Kd.
Compared with Ab12, Abs with faster off-rates (12.6, 12.70, and 12.56) are more effective, whereas Ab12.17 with a slower offrate is less effective at supporting the formation of erythroid colonies from hematopoietic precursor cells. rHu- EPO was used as a positive control.
Lacy, S. E., DeVries, P. J., Xie, N., Fung, E., Lesniewski, R. R., & Reilly, E. B. (2008). The potency of erythropoietin-mimic antibodies correlates inversely with affinity. The Journal of Immunology, 181(2), 1282-1287.
FC
Figure 3 Off-rate FACS of mutagenic Ab12 scFv library H2-1–3.
The Ab12 scFv control indicates the number and fluorescence of cells binding soluble EPOR after a 20-min competition (FL2 axis) as well as the Ab12 scFv expression level (FL1).
Lacy, S. E., DeVries, P. J., Xie, N., Fung, E., Lesniewski, R. R., & Reilly, E. B. (2008). The potency of erythropoietin-mimic antibodies correlates inversely with affinity. The Journal of Immunology, 181(2), 1282-1287.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human EPOR
- Species Reactivity
- Human
- Clone
- Ab12
- Applications
- Activ, FC, ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The EPOR antibody has been reported in applications of Activation, Flow Cytometry, Enzyme-linked Immunosorbent Assay.
FC: Approximately 6 × 10⁶ cells from each library were incubated with 0.5 μM EPOR ECD at 37°C until equilibrium was reached (2 h). Cells were then chilled on ice, washed three times with BSS at 4°C, and a 10,000-fold molar excess (5 μg/ml) of Abl2 IgG1 prewarmed to 37°C was added. After incubation for 20 min at 37°C, cells were chilled, washed, and labeled with mAb307 plus rabbit anti-6-His Ab (Research Diagnostics) for 30 min on ice. Cells were washed three times with BSS at 4°C and then incubated with a mixture of anti-mouse PE Ab and goat anti-rabbit FITC.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-040 | Human Anti-EPOR Recombinant Antibody (clone ABT-007) | WB, FuncS | Human IgG |
| HPAB-0490-CN | Mouse Anti-EPOR Recombinant Antibody (clone 21.3.1) | ELISA, WB, ICC, IHC, IP | Mouse IgG |
| HPAB-0491-CN | Mouse Anti-EPOR Recombinant Antibody (clone 19.1.2) | ELISA, WB, ICC, IHC, IP | Mouse IgG |
| HPAB-1693-FY | Human Anti-EPOR Recombinant Antibody (clone Ab12.6) | FC, ELISA | Human IgG1 |
| HPAB-1694-FY | Human Anti-EPOR Recombinant Antibody (clone Ab12.56) | FC, ELISA | Human IgG1 |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-007WM | Anti-Human EPOR Recombinant Antibody (AB-ABT2-XG2-012) | ELISA | Human Antibody |
| TAB-007WM-S(P) | Anti-Human EPOR Recombinant Antibody scFv Fragment (AB-ABT2-XG2-012) | ELISA | Human Antibody |
| TAB-007WM-F(E) | Anti-Human EPOR Recombinant Antibody Fab Fragment (AB-ABT2-XG2-012) | ELISA | Human Antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-008WM | Anti-Human EPOR Recombinant Antibody (1E3) | ELISA, WB, FC | |
| TAB-008WM-S(P) | Anti-Human EPOR Recombinant Antibody scFv Fragment (1E3) | ELISA, WB, FC | |
| TAB-009WM-S(P) | Mouse Anti-EPOR Recombinant Antibody; scFv Fragment (TAB-009WM-S(P)) | WB, FC | Mouse scFv |
| TAB-008WM-F(E) | Anti-Human EPOR Recombinant Antibody Fab Fragment (1E3) | ELISA, WB, FC | |
| TAB-009WM-F(E) | Mouse Anti-EPOR Recombinant Antibody; Fab Fragment (TAB-009WM-F(E)) | WB, FC | Mouse Fab |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-736CQ | Mouse Anti-EPOR Recombinant Antibody (clone CBL641) | Neut | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1173 | Hi-Affi™ Rabbit Anti-EPOR Recombinant Antibody (clone DS1173AB) | IP, WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0490-CN-F(E) | Mouse Anti-EPOR Recombinant Antibody (clone 21.3.1); Fab Fragment | ELISA, WB, ICC, IHC, IP | Mouse Fab |
| HPAB-0491-CN-F(E) | Mouse Anti-EPOR Recombinant Antibody (clone 19.1.2); Fab Fragment | ELISA, WB, ICC, IHC, IP | Mouse Fab |
| HPAB-1692-FY-S(P) | Recombinant Human Anti-EPOR Antibody Fab Fragment (Ab12) | FC, ELISA | Human Fab |
| HPAB-1693-FY-S(P) | Human Anti-EPOR Recombinant Antibody (clone Ab12.6); Fab Fragment | FC, ELISA | Human Fab |
| HPAB-1694-FY-S(P) | Human Anti-EPOR Recombinant Antibody (clone Ab12.56); Fab Fragment | FC, ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1692-FY-F(E) | Recombinant Human Anti-EPOR Antibody scFv Fragment (Ab12) | FC, ELISA | Human scFv |
| HPAB-1693-FY-F(E) | Human Anti-EPOR Recombinant Antibody (clone Ab12.6); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-1694-FY-F(E) | Human Anti-EPOR Recombinant Antibody (clone Ab12.56); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-1306WJ-S(P) | Mouse Anti-EPOR Recombinant Antibody (clone 3G9); scFv Fragment | ELISA, WB, Agonist | Mouse scFv |
| HPAB-1307WJ-S(P) | Human Anti-EPOR Recombinant Antibody (clone 3G9HZLC); scFv Fragment | ELISA, WB, Agonist | Humanized scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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