Recombinant Human Anti-EPOR Antibody (Ab12)
CAT#: HPAB-1692-FY
This product is a recombinant antibody that recognizes EPOR. The monoclonal antibody Ab12 reacts with the epitope of EPOR.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/77654/1825_E6_47_cr5ac349555a69b_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187266-EPOR
Normal Tissue
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77654/1825_E6_47_cr5ac349555a69b_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187266-EPOR
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human EPOR
- Species Reactivity
- Human
- Clone
- Ab12
- Applications
- Activ, FC, ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The EPOR antibody has been reported in applications of Activation, Flow Cytometry, Enzyme-linked Immunosorbent Assay.
FC: Approximately 6 × 10⁶ cells from each library were incubated with 0.5 μM EPOR ECD at 37°C until equilibrium was reached (2 h). Cells were then chilled on ice, washed three times with BSS at 4°C, and a 10,000-fold molar excess (5 μg/ml) of Abl2 IgG1 prewarmed to 37°C was added. After incubation for 20 min at 37°C, cells were chilled, washed, and labeled with mAb307 plus rabbit anti-6-His Ab (Research Diagnostics) for 30 min on ice. Cells were washed three times with BSS at 4°C and then incubated with a mixture of anti-mouse PE Ab and goat anti-rabbit FITC.
Target
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Excellent Detection
Compared to other antibodies we've tried, this antibody provides the highest signal clarity and intensity. It is now a necessary component of our immunofluorescence tests.
Robust and Stable
The antibody is strong and continues to yield consistent findings even after being stored for several months. To preserve its effectiveness, aliquoting is strongly advised.
Great for Multiplex Assays
This antibody behaves brilliantly in multiplex experiments when combined with other human antibodies, and there are no problems with cross-reactivity.
Q&As
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Are there special handling instructions for this antibody?
A: To prevent denaturation, handle the antibody with gentleness. Before using, let it defrost on ice and stir carefully. By aliquoting the antibody, many freeze-thaw cycles can be avoided.
-
How long can this antibody be stored at 4°C?
A: The antibody has a maximum shelf life of two weeks at 4°C. To store something for a longer period of time, it should be kept at -20°C.
-
What buffers are recommended for the dilution of this antibody?
A: Dilution buffers usually include PBS or TBS with a small percentage of BSA or other stabilizers. Check the datasheet for any specific recommendations.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone Ab12"
- CAT
- Product Name
See other products for "EPOR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-040 | Human Anti-EPOR Recombinant Antibody (clone ABT-007); Fab Fragment | WB, FuncS | Human Fab |
| HPAB-0490-CN-F(E) | Mouse Anti-EPOR Recombinant Antibody (clone 21.3.1); Fab Fragment | ELISA, WB, ICC, IHC, IP | Mouse Fab |
| HPAB-0491-CN-F(E) | Mouse Anti-EPOR Recombinant Antibody (clone 19.1.2); Fab Fragment | ELISA, WB, ICC, IHC, IP | Mouse Fab |
| HPAB-0169CQ-F(E) | Human Anti-EPOR Recombinant Antibody (clone ABT2-SCX-012); Fab Fragment | Agonist, ELISA | Human Fab |
| HPAB-1850-FY-S(P) | Human Anti-EPOR Recombinant Antibody (clone Ab198); Fab Fragment | Activ | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-007WM | Anti-Human EPOR Recombinant Antibody (AB-ABT2-XG2-012) | ELISA | Human Antibody |
| TAB-007WM-S(P) | Anti-Human EPOR Recombinant Antibody scFv Fragment (AB-ABT2-XG2-012) | ELISA | Human Antibody |
| TAB-007WM-F(E) | Anti-Human EPOR Recombinant Antibody Fab Fragment (AB-ABT2-XG2-012) | ELISA | Human Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-008WM | Anti-Human EPOR Recombinant Antibody (1E3) | ELISA, WB, FC | |
| TAB-008WM-S(P) | Anti-Human EPOR Recombinant Antibody scFv Fragment (1E3) | ELISA, WB, FC | |
| TAB-009WM-S(P) | Mouse Anti-EPOR Recombinant Antibody; scFv Fragment (TAB-009WM-S(P)) | WB, FC | Mouse scFv |
| TAB-008WM-F(E) | Anti-Human EPOR Recombinant Antibody Fab Fragment (1E3) | ELISA, WB, FC | |
| TAB-009WM-F(E) | Mouse Anti-EPOR Recombinant Antibody; Fab Fragment (TAB-009WM-F(E)) | WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-736CQ | Mouse Anti-EPOR Recombinant Antibody (clone CBL641) | Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1173 | Hi-Affi™ Rabbit Anti-EPOR Recombinant Antibody (clone DS1173AB) | IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0490-CN | Mouse Anti-EPOR Recombinant Antibody (clone 21.3.1) | ELISA, WB, ICC, IHC, IP | Mouse IgG |
| HPAB-0491-CN | Mouse Anti-EPOR Recombinant Antibody (clone 19.1.2) | ELISA, WB, ICC, IHC, IP | Mouse IgG |
| HPAB-0169CQ | Human Anti-EPOR Recombinant Antibody (clone ABT2-SCX-012) | Agonist, ELISA | Human IgG |
| HPAB-0359-YJ | Human Anti-EPOR Recombinant Antibody (HPAB-0359-YJ) | ELISA, FC, Activ, FuncS | Human IgG2 |
| HPAB-0360-YJ | Human Anti-EPOR Recombinant Antibody (HPAB-0360-YJ) | ELISA, FC, Activ, FuncS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0490-CN-S(P) | Mouse Anti-EPOR Recombinant Antibody (clone 21.3.1); scFv Fragment | ELISA, WB, ICC, IHC, IP | Mouse scFv |
| HPAB-0491-CN-S(P) | Mouse Anti-EPOR Recombinant Antibody (clone 19.1.2); scFv Fragment | ELISA, WB, ICC, IHC, IP | Mouse scFv |
| HPAB-0359-YJ-S(P) | Human Anti-EPOR Recombinant Antibody; scFv Fragment (HPAB-0359-YJ-S(P)) | ELISA, FC, Activ, FuncS | Human scFv |
| HPAB-0360-YJ-S(P) | Human Anti-EPOR Recombinant Antibody; scFv Fragment (HPAB-0360-YJ-S(P)) | ELISA, FC, Activ, FuncS | Human scFv |
| HPAB-1850-FY-F(E) | Human Anti-EPOR Recombinant Antibody (clone Ab198); scFv Fragment | Activ | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY90 | AbPlus™ Anti-EPOR Magnetic Beads (21.3.1) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY17 | Human Anti-EpoR (clone 10) scFv-Fc Chimera | ELISA, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC620 | Recombinant Anti-EPOR Vesicular Antibody, EV Displayed (VS-0425-YC620) | Agonist, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC70 | Recombinant Anti-EPOR (AA 347-371 x AA 382-402) Biparatopic Antibody, Tandem scFv (Clone 21.3.1 x Clone 19.1.2) | ELISA, ICC, IHC, IP, WB | Tandem scFv |
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