Mouse Anti-FCGR2A Recombinant Antibody (clone MDE-9)
CAT#: VS11-QX66
This product is a recombinant mouse antibody that recognizes FCGR2A. This antibody can be used for the following applications: FC, IHC, ELISA, IF, Block. MDE-9, selectively recognized the FcgRIIa-H131 allotype. MDE-9 was applicable to detect FcgRIIa-H131 in both flow cytometry and immunohistochemistry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1926_F1_3_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14730/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1847_F1_2_blue_red_green.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10718/26353_B_4_4.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEL, HMC-1, K-562, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143226-FCGR2A
❮
❯
Specifications
- Immunogen
- FcgammaRIIa-H131 conjugated to BSA
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human FCGR2A
- Species Reactivity
- Human
- Clone
- MDE-9
- Applications
- FC, IHC, ELISA, IF, Block
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- Fc Fragment Of IgG Receptor IIa; Fc Fragment Of IgG, Low Affinity IIa, Receptor (CD32); Immunoglobulin G Fc Receptor II; IgG Fc Receptor II-A; Fc-Gamma-RIIa; FcRII-A; FCGR2A1; CDw32; IGFR2; FCG2
- Gene ID
- 2212
- UniProt ID
- P12318
- Long Name
- Low affinity immunoglobulin gamma Fc region receptor II-a
- Cellular Localization
- Golgi apparatus, Plasma membrane
- Function
- Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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