Rabbit Anti-FOXO3 Recombinant Antibody (clone R09-3J1) (CAT#: VS3-XY3361)
The rabbit recombinant antibody specifically recognizes FOXO3 (Forkhead box O3), which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It reacts with human, mouse and rat FOXO3. It has been detected using Western Blot, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot and Immunofluorescence is 1/500-1/1000 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63104/if_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63104/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/if_selected.jpg
Figure 3 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/ihc_selected.jpg
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_B_7_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_8_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118689-FOXO3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118689-FOXO3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat FOXO3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-3J1
- Applications
- WB, ICC, IF
- Related Disease
- Rhabdomyosarcoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
- Gene ID
- 2309
- UniProt ID
- O43524
- Long Name
- Forkhead box O3
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
- Post Translation Modifications
- The N-terminus is cleaved following import into the mitochondrion. (O43524-FOXO3_HUMAN)
Glycosylation at Asn431 (NX_O43524 [NX_O43524-1])
Modification sites at PhosphoSitePlus (O43524)
Glycosylation from GlyConnect FOXO3_HUMAN (1253)
Glycosylation from GlyGen (O43524) 10 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (9 sites)
- Protein Refseq
- XP_016866074.1; XP_016866075.1
- Function
- Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity).
Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, 16751106).
Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Gastric Cancer
Hepatocellular Carcinoma
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4019z | Mouse Anti-FOXO3 Recombinant Antibody (clone 9G10) | WB, ELISA, IF, IHC | Mouse IgG2a, κ |
| MOB-1068MZ | Recombinant Mouse Anti-Human FOXO3 Antibody | IHC-P | Mouse antibody |
| MRO-0602-CN | Rabbit Anti-FOXO3 Recombinant Antibody (clone SP0054) | WB, IF | Rabbit IgG |
| VS3-CJ151 | Rabbit Anti-FOXO3 Recombinant Antibody (VS3-CJ151) | WB, IHC | Rabbit IgG |
| VS3-WK2120 | Rabbit Anti-FOXO3 (pS235) Recombinant Antibody (clone R08-7U1) | WB, IHC-P | Rabbit IgG |
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