Mouse Anti-GDF15 Recombinant Antibody (clone 5E1A5)
CAT#: ZG-0199U
Mouse anti-GDF15 antibody (clone 5E1A5) displayed high specificity against the GDF15 protein of Human.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-GDF15 Antibody (ZG-0199U) in IHC
Immunohistochemistry of paraffin-embedded human adrenal gland using ZG-0199U in 30μg/ml dilute concentrations.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11191/1404_F3_1_selected.jpg
Normal Tissue
Figure 2 Placenta
Cytotrophoblasts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining: High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11191/27866_A_1_7.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (BEWO, Hep G2, HHSteC, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130513-GDF15
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Specifications
- Immunogen
- Recombinant GDF15 Protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human GDF15
- Species Reactivity
- Human
- Clone
- 5E1A5
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Hereditary Transthyretin Amyloidosis and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:500
Target
- Alternative Names
- PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; growth/differentiation factor 15; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PTGF-beta; macrophage inhibitory cytokine 1; non-steroidal anti-inflammatory drug-activated gene-1; placental TGF-beta; placental bone morphogenetic protein; prostate differentiation factor
- Gene ID
- 9518
- UniProt ID
- Q99988
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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