Rabbit Anti-GDF15 Recombinant Antibody (clone 8E5)
CAT#: ZG-0684J
This product is a rabbit antibody that recognizes GDF15.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-GDF15 Antibody (ZG-0684J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11191/1404_F3_1_selected.jpg
Normal Tissue
Figure 2 Placenta
Cytotrophoblasts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining: High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11191/27866_A_1_7.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (BEWO, Hep G2, HHSteC, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130513-GDF15
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Specifications
- Immunogen
- A synthesized peptide derived from human GDF15
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GDF15
- Species Reactivity
- Human
- Clone
- 8E5
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
- Related Disease
- Tissue hypoxia, inflammation, acute injury and oxidative stress
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
- Alternative Names
- PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; growth/differentiation factor 15; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PTGF-beta; macrophage inhibitory cytokine 1; non-steroidal anti-inflammatory drug-activated gene-1; placental TGF-beta; placental bone morphogenetic protein; prostate differentiation factor
- Gene ID
- 9518
- UniProt ID
- Q99988
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| MOB-391-F(E) | Recombinant Anti-human GDF15 Antibody Fab Fragment | IF, FC, FuncS | Fab |
| HPAB-0142-YJ-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-0142-YJ-F(E)) | Inhib, FuncS | Human Fab |
| HPAB-0143-YJ-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-0143-YJ-F(E)) | Inhib, FuncS | Humanized Fab |
| HPAB-M0522-YC-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-M0522-YC-F(E)) | ELISA, Block | Human Fab |
| HPAB-M0523-YC-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-M0523-YC-F(E)) | ELISA, Block | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-391-S(P) | Recombinant Anti-human GDF15 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| MHH-391-S(P) | Recombinant Human Anti-human GDF15 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| HPAB-0342-CN-S(P) | Mouse Anti-GDF15 Recombinant Antibody; scFv Fragment (HPAB-0342-CN-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-0395-YJ-S(P) | Mouse Anti-GDF15 Recombinant Antibody (clone MBM-12); scFv Fragment | ELISA, Inhib | Mouse scFv |
| HPAB-0396-YJ-S(P) | Mouse Anti-GDF15 Recombinant Antibody (clone MBM-14); scFv Fragment | ELISA, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0781CL | Anti-Human GDF15 Recombinant Antibody | ELISA, WB | Humanized antibody |
| TAB-0781CL-S(P) | Anti-Human GDF15 Recombinant Antibody scFv Fragment | ELISA, WB | Humanized antibody |
| TAB-0781CL-F(E) | Anti-Human GDF15 Recombinant Antibody Fab Fragment | ELISA, WB | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0784CL | Human Anti-GDF15 Recombinant Antibody (TAB-0784CL) | ELISA, FC | Human IgG |
| TAB-0784CL-S(P) | Human Anti-GDF15 Recombinant Antibody; scFv Fragment (TAB-0784CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0784CL-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (TAB-0784CL-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1281CL | Anti-Human GDF15 Recombinant Antibody (3B7-3-3) | ELISA | |
| TAB-1282CL | Anti-Human GDF15 Recombinant Antibody (3C7-3-1) | ELISA, FuncS, Inhib | |
| TAB-0782CL-F(E) | Anti-Human GDF15 Recombinant Antibody Fab Fragment (A10.3) | ELISA, FC | |
| TAB-0783CL-F(E) | Anti-Human GDF15 Recombinant Antibody Fab Fragment (B2.2) | ELISA, FC | |
| TAB-0785CL-F(E) | Mouse Anti-GDF15 Recombinant Antibody; Fab Fragment (TAB-0785CL-F(E)) | ELISA, FC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1421 | Hi-Affi™ Rabbit Anti-GDF15 Recombinant Antibody (clone DS1421AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0142-YJ | Human Anti-GDF15 Recombinant Antibody (HPAB-0142-YJ) | Inhib, FuncS | Chimeric (mouse/human) IgG1, κ |
| HPAB-0143-YJ | Human Anti-GDF15 Recombinant Antibody (HPAB-0143-YJ) | Inhib, FuncS | Humanized IgG1, κ |
| HPAB-0395-YJ | Mouse Anti-GDF15 Recombinant Antibody (clone MBM-12) | ELISA, Inhib | Mouse IgG |
| HPAB-0396-YJ | Mouse Anti-GDF15 Recombinant Antibody (clone MBM-14) | ELISA, Inhib | Mouse IgG |
| ZG-0199U | Mouse Anti-GDF15 Recombinant Antibody (clone 5E1A5) | ELISA, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2774 | Anti-GDF15 Immunohistochemistry Kit | IHC |
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