AibGenesis™ Rabbit Anti-GLB1 Recombinant Antibody (AibG-Rb-GL439)
CAT#: VS-0426-FY439
Established via our AI-powered antibody discovery platform, this Protein A purified Rabbit IgG targets GLB1. Validated for WB, IHC, and ELISA, it delivers high precision for researchers investigating lysosomal beta-galactosidase activity and its clinical relevance in senescence and GM1 gangliosidosis.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Thyroid gland
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1
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Specifications
- Immunogen
- Human
- Host Species
- Rabbit
- Type
- Rabbit IgG, Kappa
- Specificity
- Human GLB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-GL439
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA, Immunoprecipitation
- Conjugate
- Unconjugated, Biotin and FITC are available.
- Related Disease
- GM1 Gangliosidosis
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Buffer
- PBS, 0.05% Protective protein, 50% Glycerol
- Preservative
- 0.05% Proclin 300
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1:1000-1:5000), Immunohistochemistry (1:2000-1:10000), Immunocytochemistry/Immunofluorescence (1:200-1:1000), ELISA (1:5000-1:20000), Immunoprecipitation (1:50-1:200)
Target
- Alternative Names
- GLB1; ELNR1; Beta-galactosidase; Acid beta-galactosidase; Lactase; Elastin receptor 1
- Gene ID
- 2720
- UniProt ID
- P16278
- Long Name
- Galactosidase Beta 1
- Sequence Similarities
- Belongs to the glycosyl hydrolase 35 family.
- Cellular Localization
- Lysosome
- Post Translation Modifications
- Glycosylation at Asn26, Asn247, Asn464, Asn498, Asn542, Asn545, and Asn555
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect BGAL_HUMAN (1036)
Glycosylation from GlyGen 8 sites, 81 N-linked glycans (4 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- NP_000395.3; NP_001073279.2; NP_001129074.2; NP_001303969.2; NP_001380509.1
- Function
- AMEF strain GLB1 refers to a particular strain of Acanthamoeba, which is a free-living amoeba. Specific characteristics of the GLB1 strain, often including its pathogenic potential and genetic makeup, are important in understanding Acanthamoeba infections.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GLB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-75 | Mouse Anti-GLB1 Recombinant Antibody (clone 13R4); Fab Fragment | WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-77 | Hamster Anti-GLB1 Recombinant Antibody (clone 13R4) | WB, FC | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-361 | Recombinant Mouse Anti-β-galactosidase Antibody (13R4) | WB, ELISA, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-361 | Recombinant Mouse Anti-β-galactosidase Antibody scFv Fragment (13R4) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-361 | Recombinant Mouse Anti-β-galactosidase Antibody Fab Fragment (13R4) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0772CT | Recombinant Mouse anti-Human GLB1 Monoclonal antibody (EML397) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1450 | Hi-Affi™ Rabbit Anti-GLB1 Recombinant Antibody (clone DS1450AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY594 | Recombinant Rabbit Anti-GLB1 Antibody (clone R01-3G1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY221 | Mouse Anti-NHP GLB1 Recombinant Antibody (clone OTI1C9) | WB, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY95 | Human Anti-GLB1 scFv-Fc Chimera (VS-0325-FY95) | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC450 | CytoStream™ Rabbit Anti-GLB1 Recombinant Antibody (VS13-YC450) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2820 | Anti-GLB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS14-YC365 | AibGenesis™ Rabbit Anti-GLB1 Recombinant Antibody (AibG-Rb-LB165) | WB, IHC, ICC, IF | Rabbit IgG |
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