AibGenesis™ Rabbit Anti-GLB1 Recombinant Antibody (AibG-Rb-LB165)
CAT#: VS14-YC365
The anti-GLB1 mAb (AibG-Rb-LB165) stands as an AI-designed high-performance tool, exhibiting significant binding affinity for GLB1 in human, mouse, and rat. GLB1 (β-galactosidase) is a lysosomal enzyme that hydrolyzes terminal beta-galactose residues from gangliosides and keratan sulfate. It is a key player in sphingolipid metabolism and is widely used as a biomarker for cellular senescence.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/814_D2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8382/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/759_D2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40610/755_D2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Thyroid gland
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_B_1_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69503/154814_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170266-GLB1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- GLB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-LB165
- Applications
- Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (WB): 1/500-1/2000
Immunohistochemistry (IHC): 1/50-1/200
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GLB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-75 | Mouse Anti-GLB1 Recombinant Antibody (clone 13R4); Fab Fragment | WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-77 | Hamster Anti-GLB1 Recombinant Antibody (clone 13R4) | WB, FC | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-361 | Recombinant Mouse Anti-β-galactosidase Antibody (13R4) | WB, ELISA, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-361 | Recombinant Mouse Anti-β-galactosidase Antibody scFv Fragment (13R4) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-361 | Recombinant Mouse Anti-β-galactosidase Antibody Fab Fragment (13R4) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0772CT | Recombinant Mouse anti-Human GLB1 Monoclonal antibody (EML397) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1450 | Hi-Affi™ Rabbit Anti-GLB1 Recombinant Antibody (clone DS1450AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY594 | Recombinant Rabbit Anti-GLB1 Antibody (clone R01-3G1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY221 | Mouse Anti-NHP GLB1 Recombinant Antibody (clone OTI1C9) | WB, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY95 | Human Anti-GLB1 scFv-Fc Chimera (VS-0325-FY95) | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC450 | CytoStream™ Rabbit Anti-GLB1 Recombinant Antibody (VS13-YC450) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2820 | Anti-GLB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0426-FY439 | AibGenesis™ Rabbit Anti-GLB1 Recombinant Antibody (AibG-Rb-GL439) | WB, IHC, ICC, IF, ELISA, IP | Rabbit IgG, Kappa |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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