Human Anti-GLP1R Recombinant Antibody (clone 3F52)
CAT#: HPAB-0189CQ
This product is a recombinant human antibody that recognizes GLP1R. 3F52 specifically binds to human and cynomolgus monkey GLP-1R. 3F52 blocked the GLP-1 binding site of the ECD directly and thereby acts as a competitive antagonist of native GLP-1. mAb 3F52 functioned as a neutral antagonist.
Published Data
Tested Data
Gene Expression
Inhib
Figure 1 MAb 3F52 selectively inhibits GLP-1 but not glucagon, GIP or GLP-2 receptor.
(a,b) Receptor radioligand competition binding experiments with membranes of BHK cells stably over-expressing human GLP-1R (a) and GCGR (b), respectively. (a) Displacement of 125I-GLP-1 from GLP-1R by GLP-1 and mAb 3F52. (b) 125I-glucagon is displaced from GCGR by glucagon but not mAb 3F52. (c,d) Cell-based cAMP Flashplate assay with BHK cells stably over-expressing human GLP-1R (c), GCGR, GIPR and GLP-2R (d). (c) GLP-1 induces concentration-dependent increase of intracellular cAMP, and this can be inhibited by mAb 3F52 in a concentration-dependent manner, whereas mAb 3F52 alone is completely inactive. (d) MAb 3F52 does not reveal any influence on GCGR-, GIPR- and GLP-2R-mediated cAMP accumulation induced by EC80 concentration of the respective ligands. Values have been normalized to the response of the corresponding ligand (GCG, GIP, GLP-2) in the absence of mAb 3F52.
Hennen, S., Kodra, J. T., Soroka, V., Krogh, B. O., Wu, X., Kaastrup, P.,... & Gandhi, P. S. (2016). Structural insight into antibody-mediated antagonism of the glucagon-like peptide-1 receptor. Scientific reports, 6, 26236.
FuncS
Figure 2 Characterization of Fab 3F52 by 125I-GLP-1 competition binding of GLP-1R from different species.
(a–e) Membranes of BHK cells stably over-expressing GLP-1R specifically bind to 125I-GLP-1, which can be displaced by native GLP-1 at all tested receptor species, whereas Fab 3F52 selectively reveals displacement only at human (a) and cynomolgus monkey (b) GLP-1R, and no competition is recorded at mouse (c), rat (d) and pig (e) GLP-1R.
Hennen, S., Kodra, J. T., Soroka, V., Krogh, B. O., Wu, X., Kaastrup, P.,... & Gandhi, P. S. (2016). Structural insight into antibody-mediated antagonism of the glucagon-like peptide-1 receptor. Scientific reports, 6, 26236.
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SDS-PAGE
Figure 1 Recombinant Human Anti-GLP1R Antibody (HPAB-0189CQ) in SDS-PAGE
SDS-PAGE analysis of HPAB-0189CQ in non-reduced (Lane 1) and reduced (Lane 2) conditions.
SEC-HPLC
Figure 2 Recombinant Human Anti-GLP1R Antibody (HPAB-0189CQ) in HPLC
The purity of HPAB-0189CQ was greater than 95% as determined by SEC-HPLC.
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RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (NTERA-2, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112164-GLP1R
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Specifications
- Immunogen
- Human GLP-1R ECD
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG4, κ
- Specificity
- Human GLP1R
- Species Reactivity
- Human, Monkey
- Clone
- 3F52
- Applications
- ELISA, IHC, Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Inhibition and Functional Assay. Please see the Published Data for further information.
Target
REVIEWS AND Q&AS
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Customer Review
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Excellent for research
I have used the 3F52 antibody in several ELISA and IHC experiments focusing on GLP1R detection. The antibody consistently delivers clear signals and impressive blocking results. Detailed documentation on purity and storage made planning my experiments straightforward and reliable for research purposes.
Reliable Performance
The recombinant antibody targeting GLP1R has shown remarkable consistency in blocking GLP-1 binding. I particularly appreciate the high purity (>95% by SDS-PAGE and HPLC) and the precise instructions for storage. This has been instrumental in obtaining reproducible data across multiple independent experiments.
Q&As
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What are the primary research applications validated for the GLP1R antibody?
A: Our GLP1R antibody has been validated in applications such as ELISA, IHC, and blocking assays. It consistently demonstrates high specificity for both human and monkey GLP1R. This makes it a reliable reagent for studying receptor signaling and related molecular research assays.
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How do the purity and storage instructions enhance its research use?
A: The product exhibits over 95% purity as determined by SDS-PAGE and HPLC, ensuring minimal background. We recommend short-term storage at 4°C and long-term aliquoting at -20°C. These detailed instructions help maintain the antibody's stability and performance over multiple experiments.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0189CQ, RRID: AB_3713794)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| HPAB-0385-YC-S(P) | Mouse Anti-GLP1R Recombinant Antibody (clone m38C2); scFv Fragment | FuncS | Mouse scFv |
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