Human Anti-GM2 Recombinant Antibody (clone KM966) (CAT#: HPAB-A1367-YJ)
This product is a Chimeric (mouse/human) antibody that recognizes GM2.
Specific Inquiry
IHC
Figure 1 Immunohistological detection for GM2 ganglioside storage in neurons of the Murine Midbrain at 16 Weeks was accomplished with antibody KM966.
A 1:100 dilution of the chimeric murine-human immunoglobulin G1 (IgG1) anti-GM2 ganglioside KM966.
Woodley, E., Osmon, K. J. L., Thompson, P., Richmond, C., Chen, Z., Gray, S. J., & Walia, J. S. (2018). Efficacy of a Bicistronic Vector for Correction of Sandhoff Disease in a Mouse Model. Molecular therapy. Methods & clinical development, 12, 47-57.
ADCC
Figure 2 Anti-tumor activity of KM966 in vivo and spontaneous regression of SBC-3/ADM tumors.
Fukumoto, H., Nishio, K., Ohta, S., Hanai, N., Fukuoka, K., Ohe, Y., Sugihara, K., Kodama, T., & Saijo, N. (1999). Effect of a chimeric anti-ganglioside GM2 antibody on ganglioside GM2-expressing human solid tumors in vivo. International journal of cancer, 82(5), 759–764.
CDC
Figure 3 Complement-dependent cytolysis (CDC) activity of KM966.
Fukumoto, H., Nishio, K., Ohta, S., Hanai, N., Fukuoka, K., Ohe, Y., Sugihara, K., Kodama, T., & Saijo, N. (1999). Effect of a chimeric anti-ganglioside GM2 antibody on ganglioside GM2-expressing human solid tumors in vivo. International journal of cancer, 82(5), 759-764.
Cyt
Figure 4 Effect of KM966 MAb on CDC against human SBC-3 cells.
Hanibuchi, M., Yano, S., Nishioka, Y., Yanagawa, H., Miki, T., & Sone, S. (2000). Immunological circumvention of multiple organ metastases of multidrug resistant human small cell lung cancer cells by mouse-human chimeric anti-ganglioside GM2 antibody KM966. Clinical & experimental metastasis, 18(5), 353-360.
ELISA
Figure 5 ELISA analysis of antibodies.
Nakamura, K., Koike, M., Shitara, K., Kuwana, Y., Kiuragi, K., Igarashi, S., Hasegawa, M., & Hanai, N. (1994). Chimeric anti-ganglioside GM2 antibody with antitumor activity. Cancer research, 54(6), 1511-1516.
FuncS
Figure 6 Antitumor effects of antibodies on transplanted tumor in nude mice.
Nakamura, K., Koike, M., Shitara, K., Kuwana, Y., Kiuragi, K., Igarashi, S., Hasegawa, M., & Hanai, N. (1994). Chimeric anti-ganglioside GM2 antibody with antitumor activity. Cancer research, 54(6), 1511-1516.
FC
Figure 7 Reactivity of murine KM696 and chimeric KM966 with human tumor cell lines by flow cytometry.
Nakamura, K., Koike, M., Shitara, K., Kuwana, Y., Kiuragi, K., Igarashi, S., Hasegawa, M., & Hanai, N. (1994). Chimeric anti-ganglioside GM2 antibody with antitumor activity. Cancer research, 54(6), 1511–1516.
Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG1
- Specificity
- Human GM2
- Species Reactivity
- Human
- Clone
- KM966
- Applications
- Enzyme-linked Immunosorbent Assay, Immunofluorescence, Antibody-dependent cellular cytotoxicity, Complement-dependent cytolysis, Cytotoxicity, and Functional Assay
- Related Disease
- Tumor
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the Certificate of Analysis for the lot-specific concentration.
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody can be used in Enzyme-linked Immunosorbent Assay, Immunofluorescence, Antibody-dependent cellular cytotoxicity, Complement-dependent cytolysis, Cytotoxicity, and Functional Assay. Optimal working dilutions should be determined experimentally by the investigator.
Target
- Alternative Names
- Ganglioside GM2; GM2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "GM2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-406 | Recombinant Anti-GM2 Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-406 | Recombinant Human Anti-GM2 Antibody | WB, Neut, FuncS | IgG |
| HPAB-S0039-YC | Human Anti-GM2 Recombinant Antibody (clone KM8966) | ELISA, ADCC | Human IgG |
| HPAB-S0040-YC | Human Anti-GM2 Recombinant Antibody (clone KM8967) | ELISA, ADCC | Human IgG |
| FAMAB-0054WJ | Human Anti-GM2 Recombinant Antibody (clone L55-81) | WB, ELISA, Cyt, IHC | Human IgM |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-406-F(E) | Recombinant Anti-GM2 Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-406-F(E) | Recombinant Human Anti-GM2 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| HPAB-S0040-YC-F(E) | Human Anti-GM2 Recombinant Antibody (clone KM8967); Fab Fragment | ELISA, ADCC | Human Fab |
| FAMAB-0054WJ-F(E) | Human Anti-GM2 Recombinant Antibody (clone L55-81); Fab Fragment | WB | Human Fab |
| MOB-102YJL-F(E) | Mouse Anti-GM2 Recombinant Antibody (clone KM796); Fab Fragment | ELISA, ADCC, CDC, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-406-S(P) | Recombinant Human Anti-GM2 Antibody scFv Fragment | WB, IF, FuncS | scFv |
| HPAB-S0039-YC-S(P) | Human Anti-GM2 Recombinant Antibody (clone KM8966); scFv Fragment | ELISA, ADCC | Human scFv |
| HPAB-S0040-YC-S(P) | Human Anti-GM2 Recombinant Antibody (clone KM8967); scFv Fragment | ELISA, ADCC | Human scFv |
| FAMAB-0054WJ-S(P) | Human Anti-GM2 Recombinant Antibody (clone L55-81); scFv Fragment | WB | Human scFv |
| MOB-102YJL-S(P) | Mouse Anti-GM2 Recombinant Antibody (clone KM796); scFv Fragment | ELISA, ADCC, CDC, FuncS | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-320CL | Anti-Human GM2 Recombinant Antibody (BIW-8962) | FC, FuncS, IF | Antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-169CT | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody (DMF10.62.3) | ELISA, FC, IHC | |
| TAB-169CT-S(P) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody scFv Fragment (DMF10.62.3) | ELISA, FC, IHC | |
| TAB-170CT-S(P) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody scFv Fragment (DMF10.167.4) | ELISA, FC, IHC | |
| TAB-169CT-F(E) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody Fab Fragment (DMF10.62.3) | ELISA, FC, IHC | |
| TAB-170CT-F(E) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody Fab Fragment (DMF10.167.4) | ELISA, FC, IHC |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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