Rabbit Anti-GPA33 Recombinant Antibody (clone 4H4)
CAT#: ZG-0114U
Rabbit anti-GPA33 antibody (clone 4H4) displayed high specificity against the GPA33 protein of Human.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-GPA33 Antibody (ZG-0114U) in IHC
IHC image of ZG-0114U diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Normal Tissue
Figure 1 High expression in colon
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/ihc_selected.jpg
Normal Tissue
Figure 2 Low expression in liver
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_7_4_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_9_3.jpg
Normal Tissue
Figure 4 Appendix
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_2_2.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143167-GPA33
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Specifications
- Immunogen
- A synthesized peptide derived from human GPA33
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GPA33
- Species Reactivity
- Human
- Clone
- 4H4
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Foodborne Botulism and Wound Botulism
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| HPAB-1718-FY-F(E) | Human Anti-GPA33 Recombinant Antibody (clone 125M10AA); scFv Fragment | ELISA | Human scFv |
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| HPAB-0522-YJ-S(P) | Human Anti-GPA33 Recombinant Antibody; scFv Fragment (HPAB-0522-YJ-S(P)) | WB, IP, FC, ELISA, FuncS | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1717-FY-S(P) | Human Anti-GPA33 Recombinant Antibody (clone 263A17); Fab Fragment | ELISA | Human Fab |
| HPAB-1718-FY-S(P) | Human Anti-GPA33 Recombinant Antibody (clone 125M10AA); Fab Fragment | ELISA | Human Fab |
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| HPAB-0521-YJ-F(E) | Human Anti-GPA33 Recombinant Antibody; Fab Fragment (HPAB-0521-YJ-F(E)) | WB, IP, FC, ELISA, FuncS | Chimeric (Mouse/Human) Fab |
| HPAB-0522-YJ-F(E) | Human Anti-GPA33 Recombinant Antibody; Fab Fragment (HPAB-0522-YJ-F(E)) | WB, IP, FC, ELISA, FuncS | Humanized Fab |
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| VS-0425-YC555 | Recombinant Anti-GPA33 Vesicular Antibody, EV Displayed (VS-0425-YC555) | ELISA, FC, Cell-uptake |
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