Recombinant Mouse Anti-HA Antibody Fab Fragment (H153-70A12)

Customer Review

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Emily Hill
29.Nov,23

Ideal for HA Detection

The Anti-HA Antibody Fab Fragment has delivered excellent results in our detection assays. The specificity and sensitivity for HA are top-notch, providing clear and accurate detection. This antibody has greatly improved our detection studies and made our experiments more reliable.

James Carter
26.Jul,22

High Specificity and Sensitivity

We incorporated this antibody into our Western blot and immunoprecipitation assays, and it has been a great addition. The specificity and sensitivity for HA are outstanding, consistently detecting the target protein with high clarity. The reliable performance has made it an essential tool in our research.

Rachel Perez
15.Jun,21

Consistent and Reproducible

This antibody has proven to be incredibly consistent in various assays, including flow cytometry and immunofluorescence. The high-quality, reproducible results and strong affinity for HA have significantly benefited our experiments, making it a staple in our lab. The consistent performance has greatly enhanced our data quality.

Q&As

1. Is the anti-HA antibody Fab fragment suitable for use in Western blotting?

   A: Yes, the anti-HA antibody Fab fragment (FAMAB-0254-YC-F(E)) is suitable for use in Western blotting. It provides specific binding to HA-tagged proteins, allowing for reliable detection in Western blot assays.

2. What is the recommended dilution for using the anti-HA antibody Fab fragment in immunofluorescence?

   A: The recommended dilution for using the anti-HA antibody Fab fragment (FAMAB-0254-YC-F(E)) in immunofluorescence is typically 1:100 to 1:500. It is advisable to optimize the dilution based on the specific experimental conditions and sample types.

3. Can the anti-HA antibody Fab fragment be used in ELISA applications?

   A: Yes, the anti-HA antibody Fab fragment (FAMAB-0254-YC-F(E)) can be used in ELISA applications. It has been validated for use in such assays and provides reliable detection of HA-tagged proteins.

4. Is the anti-HA antibody Fab fragment suitable for use in immunoprecipitation assays?

   A: Yes, the anti-HA antibody Fab fragment (FAMAB-0254-YC-F(E)) is suitable for use in immunoprecipitation assays. It provides specific binding to HA-tagged proteins, enabling their successful precipitation from complex mixtures.

5. What are the storage recommendations for the anti-HA antibody Fab fragment?

   A: The recommended storage condition for the anti-HA antibody Fab fragment (FAMAB-0254-YC-F(E)) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Wang, Lixue, et al. "Bioinspired engineering of fusogen and targeting moiety equipped nanovesicles." Nature Communications 14.1 (2023): 3366. https://doi.org/s41467-023-39181-2
   This study focuses on the bioinspired engineering of fusogen and targeting moiety equipped nanovesicles (eFT-CNVs) to enhance drug delivery efficiency. The researchers developed eFT-CNVs by mechanically extruding genetically modified donor cells to produce high-yield and consistent nanovesicles. These engineered vesicles co-express a fusogen derived from the Sindbis virus and an anti-GPC3 single-chain variable fragment (scFv), enabling them to selectively bind to GPC3-overexpressing cancer cells and facilitate efficient cytosolic drug delivery through membrane fusion. The study demonstrated that eFT-CNVs significantly improve the treatment efficacy of drugs targeting cytosolic components, suggesting that these nanovesicles hold promise for advancing nanomedicine and precision medicine.
   Creative Biolabs contributed to this research by providing the anti-HA antibody Fab fragment (Cat# FAMAB-0254-YC-F(E)). This antibody was essential for estimating the concentration of fusogen on eFT-CNVs, which was crucial for evaluating the efficiency of membrane fusion and drug delivery. By enabling precise quantification of fusogen molecules, the anti-HA antibody from Creative Biolabs supported the researchers in optimizing the design and functionality of the bioengineered nanovesicles, thereby facilitating the study's goal of enhancing targeted drug delivery systems.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# FAMAB-0254-YC-F(E), RRID: AB_3111324)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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