Human Anti-HBEGF Recombinant Antibody (clone KM3966) (CAT#: VS-0624-FY12)
This is a human recombinant monoclonal antibody that binds to human HB-EGF. It shows no cross reaction with mouse HB-EGF. Anti-HB-EGF chimeric antibody KM3966 exhibits antibody-dependent cellular cytotoxicity against HB-EGF-positive human solid cancer cell lines. It can be used in Neut, ADCC, FC assays.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/ihc_selected.jpg

(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_B_8_5.jpg

(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_A_8_3.jpg

(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_A_9_5.jpg

(Cardiomyocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_B_6_6.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113070-HBEGF
Specifications
- Immunogen
- Recombinant human HB-EGF
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human HBEGF
- Species Reactivity
- Human
- Clone
- KM3966
- Applications
- Neutralization, ADCC, Flow Cytometry
- Related Disease
- Diphtheria and Perinatal Necrotizing Enterocolitis
Product Property
- Clonality
- Monoclonal Antibody
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Neutralization: 30 μg/mL
Target
- Alternative Names
- Heparin Binding EGF Like Growth Factor; Diphtheria Toxin Receptor (Heparin-Binding Epidermal Growth Factor-Like Growth Factor); Diphtheria Toxin Receptor (Heparin-Binding EGF-Like Growth Factor); Heparin-Binding Epidermal Growth Factor; HEGFL; DTR
- Gene ID
- 1839
- UniProt ID
- Q99075
- Long Name
- Heparin Binding EGF Like Growth Factor
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Several N-termini have been identified by direct sequencing.
The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
(Q99075-HBEGF_HUMAN)
O-glycosylated with core 1 or possibly core 8 glycans.
Thr-47 is a minor glycosylation site compared to Thr-44.
(Q99075-HBEGF_HUMAN)
Glycosylation at Thr37, Ser38, Thr44, Ser46, Thr47, Thr75, Ser77, Ser78, and Thr85 (NX_Q99075 [NX_Q99075-1])
Modification sites at PhosphoSitePlus (Q99075)
Glycosylation from GlyConnect HBEGF_HUMAN (809)
Glycosylation from GlyGen (Q99075) 10 sites, 7 O-linked glycans (9 sites)
- Protein Refseq
- NP_001936.1
- Function
- May be involved in macrophage-mediated cellular proliferation.
It is mitogenic for fibroblasts, but not endothelial cells.
It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF.
Also acts as a diphtheria toxin receptor.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HBEGF"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-340CL | Anti-Human HBEGF Recombinant Antibody (KHK2866) | FuncS | Antibody |
TAB-015CT | Anti-Human HB-EGF Recombinant Antibody | ELISA, Inhibition, FC | Humanized antibody |
TAB-015CT-S(P) | Anti-Human HB-EGF Recombinant Antibody scFv Fragment | ELISA, Inhibition, FC | Humanized antibody |
TAB-015CT-F(E) | Anti-Human HB-EGF Recombinant Antibody Fab Fragment | ELISA, Inhibition, FC | Humanized antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-0624-FY12. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, IHC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: WB, IHC, FC, Cyt, ELISA
Application: ELISA
Application: ELISA, IHC, FC, WB, IF, IA
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.