Human Anti-HBEGF Recombinant Antibody (clone KM3966)
CAT#: VS-0624-FY12
This is a human recombinant monoclonal antibody that binds to human HB-EGF. It shows no cross reaction with mouse HB-EGF. Anti-HB-EGF chimeric antibody KM3966 exhibits antibody-dependent cellular cytotoxicity against HB-EGF-positive human solid cancer cell lines. It can be used in Neut, ADCC, FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_A_9_5.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_B_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HBF TERT88, JURKAT, TIME, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113070-HBEGF
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Specifications
- Immunogen
- Recombinant human HB-EGF
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human HBEGF
- Species Reactivity
- Human
- Clone
- KM3966
- Applications
- Neutralization, ADCC, Flow Cytometry
- Related Disease
- Diphtheria and Perinatal Necrotizing Enterocolitis
Product Property
- Clonality
- Monoclonal Antibody
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Neutralization: 30 μg/mL
Target
- Alternative Names
- Heparin Binding EGF Like Growth Factor; Diphtheria Toxin Receptor (Heparin-Binding Epidermal Growth Factor-Like Growth Factor); Diphtheria Toxin Receptor (Heparin-Binding EGF-Like Growth Factor); Heparin-Binding Epidermal Growth Factor; HEGFL; DTR
- Gene ID
- 1839
- UniProt ID
- Q99075
- Long Name
- Heparin Binding EGF Like Growth Factor
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Several N-termini have been identified by direct sequencing.
The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
(Q99075-HBEGF_HUMAN)
O-glycosylated with core 1 or possibly core 8 glycans.
Thr-47 is a minor glycosylation site compared to Thr-44.
(Q99075-HBEGF_HUMAN)
Glycosylation at Thr37, Ser38, Thr44, Ser46, Thr47, Thr75, Ser77, Ser78, and Thr85 (NX_Q99075 [NX_Q99075-1])
Modification sites at PhosphoSitePlus (Q99075)
Glycosylation from GlyConnect HBEGF_HUMAN (809)
Glycosylation from GlyGen (Q99075) 10 sites, 7 O-linked glycans (9 sites)
- Protein Refseq
- NP_001936.1
- Function
- May be involved in macrophage-mediated cellular proliferation.
It is mitogenic for fibroblasts, but not endothelial cells.
It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF.
Also acts as a diphtheria toxin receptor.
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Related Diseases
Bladder Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HBEGF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1493z | Mouse Anti-HBEGF Recombinant Antibody (clone 25C6) | WB, IHC, Neut | Mouse IgG1 |
| HPAB-0119-YC | Mouse Anti-HBEGF Recombinant Antibody (clone 2F7) | ELISA, FC, WB, Neut | Mouse IgG |
| HPAB-0593-CN | Human Anti-HBEGF Recombinant Antibody (HPAB-0593-CN) | WB, ELISA, FC, Neut | Chimeric (mouse/human) IgG1 |
| HPAB-0594-CN | Human Anti-HBEGF Recombinant Antibody (HPAB-0594-CN) | WB, ELISA, FC, Neut | Humanized IgG1 |
| VS3-FY656 | Recombinant Rabbit Anti-HBEGF Antibody (clone R08-9C2) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-340CL | Anti-Human HBEGF Recombinant Antibody (KHK2866) | FuncS | Antibody |
| TAB-015CT | Anti-Human HB-EGF Recombinant Antibody | ELISA, Inhibition, FC | Humanized antibody |
| TAB-015CT-S(P) | Anti-Human HB-EGF Recombinant Antibody scFv Fragment | ELISA, Inhibition, FC | Humanized antibody |
| TAB-015CT-F(E) | Anti-Human HB-EGF Recombinant Antibody Fab Fragment | ELISA, Inhibition, FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-895CQ | Mouse Anti-HBEGF Recombinant Antibody (clone MM0324-3R44) | WB, Neut | Mouse IgG2 |
| NEUT-896CQ | Mouse Anti-HBEGF Recombinant Antibody (clone CBL069) | WB, Neut | Mouse IgG2 |
| NEUT-897CQ | Mouse Anti-HBEGF Recombinant Antibody (clone CBL271) | WB, Neut, ELISA | Mouse IgG2a |
| NEUT-898CQ | Mouse Anti-HBEGF Recombinant Antibody (clone CBL374) | Neut, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0119-YC-S(P) | Mouse Anti-HBEGF Recombinant Antibody (clone 2F7); scFv Fragment | ELISA, FC, WB, Neut | Mouse scFv |
| HPAB-0593-CN-S(P) | Mouse Anti-HBEGF Recombinant Antibody; scFv Fragment (HPAB-0593-CN-S(P)) | WB, ELISA, FC, Neut | Mouse scFv |
| HPAB-0594-CN-S(P) | Human Anti-HBEGF Recombinant Antibody; scFv Fragment (HPAB-0594-CN-S(P)) | WB, ELISA, FC, Neut | Humanized scFv |
| HPAB-0091-LS-S(P) | Mouse Anti-HBEGF Recombinant Antibody (clone HA-20); scFv Fragment | Inhib, FC, ELISA | Mouse scFv |
| HPAB-0092-LS-S(P) | Mouse Anti-HBEGF Recombinant Antibody (clone HB-20); scFv Fragment | Inhib, FC, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0119-YC-F(E) | Mouse Anti-HBEGF Recombinant Antibody (clone 2F7); Fab Fragment | ELISA, FC, WB, Neut | Mouse Fab |
| HPAB-0593-CN-F(E) | Human Anti-HBEGF Recombinant Antibody; Fab Fragment (HPAB-0593-CN-F(E)) | WB, ELISA, FC, Neut | Human Fab |
| HPAB-0594-CN-F(E) | Human Anti-HBEGF Recombinant Antibody; Fab Fragment (HPAB-0594-CN-F(E)) | WB, ELISA, FC, Neut | Humanized Fab |
| HPAB-0091-LS-F(E) | Mouse Anti-HBEGF Recombinant Antibody (clone HA-20); Fab Fragment | Inhib, FC, ELISA | Mouse Fab |
| HPAB-0092-LS-F(E) | Mouse Anti-HBEGF Recombinant Antibody (clone HB-20); Fab Fragment | Inhib, FC, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0387-YC | Recombinant Camelid Anti-HBEGF Single Domain Antibody (HPAB-M0387-YC) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC629 | Recombinant Anti-HBEGF Vesicular Antibody, EV Displayed (VS-0425-YC629) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3071 | Anti-HBEGF Immunohistochemistry Kit | IHC |
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