Mouse Anti-HBEGF Recombinant Antibody (clone 2F7)
CAT#: HPAB-0119-YC
Described are cross-specific antibody molecules with binding specificity for HBEGF. The antibody molecules may be used in methods of treatment of cancer and diseases associated with angiogenesis.
Published Data
Gene Expression
Inhib
Figure 1 Inhibition of ligand stimulation phosphorylation of EGFR in MDA-MB231 cells and HCT116 cells.
Cells were serum starved before addition of antibody and ligand. Cross-specific antibody (8d7 1f6 2f7, i.e. "2F7") was pre-incubated with recombinant HBEGF for 1 hour before addition to cells. Cells were stimulated for 20 mins before preparation of cell lysates. 30 μg of protein was loaded on SDS-PAGE gel and transferred to nitrocellulose membrane. The membrane was probed with anti pEGFR(Tyr1068)
Inhib
Figure 2 Inhibition of ligand stimulation phosphorylation of EGFR in LoVo cells.
Cells were serum starved before addition of antibody and ligand. Cross-specific antibody (8d7 1f6 2f7, i.e. "2F7") was pre-incubated with recombinant HBEGF for 1 hour before addition to cells. Cells were stimulated for 20 mins before preparation of cell lysates. 30 μg of protein was loaded on SDS-PAGE gel and transferred to nitrocellulose membrane. The membrane was probed with anti pEGFR(Tyr 1068)
Migration assay
Figure 3 The results of a migration assay on MDA-231 breast carcinoma cell line assessed in the presence of 300 nM cross-specific monoclonal antibodies (8d7 1f6 2f7 i.e. “2F7” or 8d7 1c8 1e9 i.e. “1E9”) or isotype control.
The assay was left for 17 hrs before wound width was measured. The right hand panels showing representative images obtained in the absence of treatment and in the presence of 8d7 1f6 2b3 i.e. "2B3"
Invasion assay
Figure 4 The results of an invasion assay of HUVEC cells through matrigel in the presence of 500 nM Bi-specific monoclonal antibody ((8d7 1f6 2f7 i.e. “2F7” or 8d7 1c8 1e9 i.e. “1E9” and negative control antibodies.
The cells were left for 24 hours before being stained by Hoescht and number of invaded cells per field of view counted.
Figure 5 Tube assay analysis of cross-specific antibody.
Huvec cells were seeded in the presence of cross-specific antibodies or control antibodies in wells coated with matrigel. Tube structure was analysed after 17 hrs. Images were taken and branching points around nodes counted. The lower panels show representative images with the upper panel illustrating in barchart format the number of branching points around nodes for untreated cells, cells treated with a control antibody and cells treated with the 8d7 1f6 2f7 antibodies.
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Normal Tissue
Figure 1 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_A_9_5.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53243/120132_B_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HBF TERT88, JURKAT, TIME, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113070-HBEGF
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HB-EGF
- Species Reactivity
- Human
- Clone
- 2F7
- Applications
- ELISA, FC, WB, Neut
- Related Disease
- Cancer, angiogenesis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Inhibition, Migration assay and Invasion assay.
Target
REVIEWS AND Q&AS
CITATIONS
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Responsive Customer Support
My questions on the application of the antibody were answered promptly by the customer service team. Excellent assistance!
User-Friendly Protocols
The experimental methods went smoothly and effectively because of the supplied protocols, which were straightforward to understand and follow.
Stable and Reliable
Even after multiple freeze-thaw cycles, this antibody held its stability. It produced consistent outcomes with no decrease in activity.
Q&As
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What supporting information is included with the product?
A: A thorough datasheet including application notes, storage guidelines, and validation data is included with the product.
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What size aliquots are available for purchase?
A: Typically, standard aliquots are packaged in 1 mg vials. On request, custom sizes can be available.
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Are there any publications supporting the use of this antibody?
A: Yes, the usage of this antibody is mentioned in a number of peer-reviewed papers. References for citations are available upon request.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Bladder Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 2F7"
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- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1493z | Mouse Anti-HBEGF Recombinant Antibody (clone 25C6) | WB, IHC, Neut | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-340CL | Anti-Human HBEGF Recombinant Antibody (KHK2866) | FuncS | Antibody |
| TAB-015CT | Anti-Human HB-EGF Recombinant Antibody | ELISA, Inhibition, FC | Humanized antibody |
| TAB-015CT-S(P) | Anti-Human HB-EGF Recombinant Antibody scFv Fragment | ELISA, Inhibition, FC | Humanized antibody |
| TAB-015CT-F(E) | Anti-Human HB-EGF Recombinant Antibody Fab Fragment | ELISA, Inhibition, FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-895CQ | Mouse Anti-HBEGF Recombinant Antibody (clone MM0324-3R44) | WB, Neut | Mouse IgG2 |
| NEUT-896CQ | Mouse Anti-HBEGF Recombinant Antibody (clone CBL069) | WB, Neut | Mouse IgG2 |
| NEUT-897CQ | Mouse Anti-HBEGF Recombinant Antibody (clone CBL271) | WB, Neut, ELISA | Mouse IgG2a |
| NEUT-898CQ | Mouse Anti-HBEGF Recombinant Antibody (clone CBL374) | Neut, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0119-YC-S(P) | Mouse Anti-HBEGF Recombinant Antibody (clone 2F7); scFv Fragment | ELISA, FC, WB, Neut | Mouse scFv |
| HPAB-0593-CN-S(P) | Mouse Anti-HBEGF Recombinant Antibody; scFv Fragment (HPAB-0593-CN-S(P)) | WB, ELISA, FC, Neut | Mouse scFv |
| HPAB-0594-CN-S(P) | Human Anti-HBEGF Recombinant Antibody; scFv Fragment (HPAB-0594-CN-S(P)) | WB, ELISA, FC, Neut | Humanized scFv |
| HPAB-0091-LS-S(P) | Mouse Anti-HBEGF Recombinant Antibody (clone HA-20); scFv Fragment | Inhib, FC, ELISA | Mouse scFv |
| HPAB-0092-LS-S(P) | Mouse Anti-HBEGF Recombinant Antibody (clone HB-20); scFv Fragment | Inhib, FC, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0593-CN-F(E) | Human Anti-HBEGF Recombinant Antibody; Fab Fragment (HPAB-0593-CN-F(E)) | WB, ELISA, FC, Neut | Human Fab |
| HPAB-0594-CN-F(E) | Human Anti-HBEGF Recombinant Antibody; Fab Fragment (HPAB-0594-CN-F(E)) | WB, ELISA, FC, Neut | Humanized Fab |
| HPAB-0091-LS-F(E) | Mouse Anti-HBEGF Recombinant Antibody (clone HA-20); Fab Fragment | Inhib, FC, ELISA | Mouse Fab |
| HPAB-0092-LS-F(E) | Mouse Anti-HBEGF Recombinant Antibody (clone HB-20); Fab Fragment | Inhib, FC, ELISA | Mouse Fab |
| HPAB-0094-LS-F(E) | Mouse Anti-HBEGF Recombinant Antibody (clone 8d71c81e9); Fab Fragment | ELISA, Inhib, WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0387-YC | Recombinant Camelid Anti-HBEGF Single Domain Antibody (HPAB-M0387-YC) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC629 | Recombinant Anti-HBEGF Vesicular Antibody, EV Displayed (VS-0425-YC629) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3071 | Anti-HBEGF Immunohistochemistry Kit | IHC |
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