Mouse Anti-HIST3H3 Recombinant Antibody (VS3-WK1018)
CAT#: VS3-WK1018
This product is a mouse monoclonal antibody that recognizes HIST3H3.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/10149_A_8_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/10149_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/10149_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/10149_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/10149_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/10149_A_4_6.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3812/10149_B_7_1.jpg
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Specifications
- Immunogen
- A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HIST3H3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IF, IP, CHIP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.3), 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C for 1 year.Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-2000), Immunohistochemistry (1:50-200), Immunofluorescence (1:50-200), Immunoprecipitation (1:20-100) and Chromatin Immunoprecipitation.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
See other products for "HIST3H3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1611 | Rabbit Anti-HIST3H3 Recombinant Antibody (clone DS1611AB) | WB, IP, IHC | Rabbit IgG |
| MOR-0034-FY | Rabbit Anti-HIST3H3 Recombinant Antibody (clone AFY0005) | ICC, IHC, WB, Inhib | Rabbit IgG |
| MOR-0041-FY | Rabbit Anti-HIST3H3 Recombinant Antibody (clone AFY0012) | ICC, IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3042 | PE-Gogo-B*0101/Human HIST3H3 (RRYQKSTEL) MHC Tetramer | FCM | |
| MHC-LC3389 | PE-B27/Human HIST3H3 (RRYQKSTEL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1984-FY | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis16) | ELISA | Human IgG |
| HPAB-1985-FY | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis18) | ELISA | Human IgG |
| VS3-QX529 | Mouse Anti-HIST3H3 Recombinant Antibody (clone 5A11E2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK1019 | Rabbit Anti-HIST3H3 Recombinant Antibody (VS3-WK1019) | WB, IHC, ICC, IP | Rabbit IgG |
| VS3-WK2203 | Mouse Anti-HIST3H3 (acetyl K9) Recombinant Antibody (VS3-WK2203) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1984-FY-F(E) | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis16); Fab Fragment | ELISA | Human Fab |
| HPAB-1985-FY-F(E) | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis18); Fab Fragment | ELISA | Human Fab |
| HPAB-1986-FY-F(E) | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis19); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1984-FY-S(P) | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis16); scFv Fragment | ELISA | Human scFv |
| HPAB-1985-FY-S(P) | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis18); scFv Fragment | ELISA | Human scFv |
| HPAB-1986-FY-S(P) | Human Anti-HIST3H3 Recombinant Antibody (clone SLEhis19); scFv Fragment | ELISA | Human scFv |
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