Human Anti-HIV-1 gp41 Recombinant Antibody (clone Md-1 (MD-1, Md1)) (CAT#: MRO-4161CQ)

Figure 1 Western blot detecting ADA.C1 Env glycoprotein with anti V3 mAb 447-52D and trimer-specific mAb Md-1.

CHO-neg corresponds to the supernatant of CHO cells transfected with empty vector and used as negative control for biopanning.

Trott, M., Weiß, S., Antoni, S., Koch, J., Von Briesen, H., Hust, M., & Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3-and a trimer-specific gp41 antibody. PLoS One, 9(5).

Figure 2 Characterization of soluble lectin purified ADA.C1 gp140 glycoproteins.

Binding of various mAbs directed against linear and conformational Env epitopes and HIV-positive sera to immobilized ADA.C1 determined by ELISA.

Trott, M., Weiß, S., Antoni, S., Koch, J., Von Briesen, H., Hust, M., & Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3-and a trimer-specific gp41 antibody. PLoS One, 9(5).

Figure 3 Immunoprecipitation of gp41 from HIV-1-infected cell lysates.

Sup-T1 cells infected with BaL were lysed and incubated with either anti-3a-C24, anti-3a-N36 antisera or anti-gp41 MAb Md-1 bound to Protein A-Sepharose beads.

Sadler, K., Zhang, Y., Xu, J., Yu, Q., & Tam, J. P. (2008). Quaternary protein mimetics of gp41 elicit neutralizing antibodies against HIV fusion‐active intermediate state. Peptide Science, 90(3), 320-329.