Mouse Anti-HLA-DRA Recombinant Antibody (clone LN3) (CAT#: VS3-CJ274)

• Immunogen
• Native purified human HLA-DR

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human HLA-DRA

• Species Reactivity
• Human

• Clone
• LN3

• Applications
• IF, FC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS

• Preservative
• 0.02% sodium azide

• Storage
• Store at -20°C for one year.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

• Alternative Names
• HLA-DRA1

• Gene ID
• 3122

• UniProt ID
• P01903

• Sequence Similarities
• Belongs to the MHC class II family.

• Cellular Localization
• Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Lysosome

• Post Translation Modifications
• Ubiquitinated by MARCHF1 or MARCHF8 at Lys-244 leading to down-regulation of MHCII. When associated with ubiquitination of the beta chain at 'Lys-254', the down-regulation of MHCII may be highly effective.

• Protein Refseq
• NP_061984.2

• Function
• An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed:29884618, PubMed:17334368, PubMed:8145819, PubMed:15322540, PubMed:22327072, PubMed:27591323, PubMed:31495665, PubMed:15265931, PubMed:9075930, PubMed:24190431).
  Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed:8145819).
  In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed:31495665).
  Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed:17182262, PubMed:23783831).
  The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed:25413013).
  The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed:8145819).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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