CytoStream™ Rabbit Anti-HSP90AB1 Recombinant Antibody (VS13-YC555)

Name: CytoStream™ Rabbit Anti-HSP90AB1 Recombinant Antibody (VS13-YC555)
SKU: VS13-YC555
Rating: 5 (1 reviews)

CAT#: VS13-YC555

The CytoStream™ Rabbit Anti-HSP90AB1 Recombinant Antibody is specific for human, mouse and rat HSP90AB1. HSP90AB1 belongs to a family of heat shock proteins aiding proper protein folding and degradation, with overexpression associated with various conditions such as gastric inflammation. The anti-HSP90AB1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/958_C6_2_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/911_A5_1_blue_red_green.jpg

Normal Tissue Figure 4 IHC staining of human placenta

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/ihc_selected.jpg

Normal Tissue Figure 5 Cerebral cortex

Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_B_7_5.jpg

Normal Tissue Figure 6 Colon

Goblet cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_4.jpg

Normal Tissue Figure 8 Kidney

Cells in tubules
Staining:MediumIntensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_7_5.jpg

Normal Tissue Figure 9 Testis

Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_6_6.jpg

Normal Tissue Figure 10 Placenta

Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_2_7.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096384-HSP90AB1

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Specifications

Immunogen
Recombinant human Hsp90 beta, aa 485-724.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat HSP90AB1

Species Reactivity
Human, Mouse, Rat

Applications
WB, IHC-P, ICC, IF, FC

Conjugate
NONE

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

Preservative
0.05% Sodium azide

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Shipping with ice pack.

Applications

Application Notes
Western blot (WB): 1/1000-1/10000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100

Target

Alternative Names
Heat Shock Protein 90 Alpha Family Class B Member 1; Heat Shock Protein 90kDa Alpha (Cytosolic), Class B Member 1; Heat Shock 90kD Protein 1, Beta; Heat Shock 84 KDa; HSP90B; HSPC2; HSPCB; HSP84; Heat Shock Protein 90kDa Alpha Family Class B Member 1;

Gene ID
3326

UniProt ID
P08238

Long Name
Heat Shock Protein 90 Alpha Family Class B Member 1

Cellular Localization
Secreted; Nucleus; Cytoplasm; Melanosome; Cell membrane; Dynein axonemal particle

Function
Signal transduction

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS13-YC555.pdf

MSDS

MSDS - VS13-YC555.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

CAT
Product Name

Isotype Control

CAT
Product Name

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VS-0525-XY3302	Anti-Human HSP90AB1 Immunohistochemistry Kit	IHC

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VS-0525-XY3304	Anti-Chicken HSP90AB1 Immunohistochemistry Kit	IHC

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