CytoStream™ Rabbit Anti-HSP90AB1 Recombinant Antibody (VS13-YC555)
CAT#: VS13-YC555
The CytoStream™ Rabbit Anti-HSP90AB1 Recombinant Antibody is specific for human, mouse and rat HSP90AB1. HSP90AB1 belongs to a family of heat shock proteins aiding proper protein folding and degradation, with overexpression associated with various conditions such as gastric inflammation. The anti-HSP90AB1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/958_C6_2_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/911_A5_1_blue_red_green.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Goblet cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:MediumIntensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_6_6.jpg
Normal Tissue
Figure 10 Placenta
Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_2_7.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096384-HSP90AB1
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Specifications
- Immunogen
- Recombinant human Hsp90 beta, aa 485-724.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HSP90AB1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/10000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Heat Shock Protein 90 Alpha Family Class B Member 1; Heat Shock Protein 90kDa Alpha (Cytosolic), Class B Member 1; Heat Shock 90kD Protein 1, Beta; Heat Shock 84 KDa; HSP90B; HSPC2; HSPCB; HSP84; Heat Shock Protein 90kDa Alpha Family Class B Member 1;
- Gene ID
- 3326
- UniProt ID
- P08238
- Long Name
- Heat Shock Protein 90 Alpha Family Class B Member 1
- Cellular Localization
- Secreted; Nucleus; Cytoplasm; Melanosome; Cell membrane; Dynein axonemal particle
- Function
- Signal transduction
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSP90AB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-516 | Recombinant Anti-human HSP90AB1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MOB-0049F | Mouse Anti-HSP90AB1 Recombinant Antibody (MOB-0049F) | WB, IHC-P, IF | Mouse IgG |
| VS7-0425-WR87 | Mouse Anti-HSP90AB1 Recombinant Antibody (clone M2) | WB, IHC-P, IF, ICC | Mouse IgG |
| VS7-0425-WR88 | Mouse Anti-HSP90AB1 Monoclonal Antibody (VS7-0425-WR88) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR89 | Mouse Anti-HSP90AB1 Monoclonal Antibody (VS7-0425-WR89) | WB, IHC-P, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-516-F(E) | Recombinant Anti-human HSP90AB1 Antibody Fab Fragment | ELISA, RIA, FuncS | Fab |
| MHH-516-F(E) | Recombinant Human Anti-human HSP90AB1 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-516-S(P) | Recombinant Anti-human HSP90AB1 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| MHH-516-S(P) | Recombinant Human Anti-human HSP90AB1 Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1690 | Rabbit Anti-HSP90AB1 Recombinant Antibody (clone DS1690AB) | WB, IHC-P, ICC, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3385 | PE-A2/Human HSP90AB1 (ILDKKVEKV) MHC Tetramer | FCM | |
| MHC-LC3387 | PE-B*27:05/Human HSP90AB1 (RRVKEVVKK) MHC Tetramer | FCM | |
| MHC-LC3388 | PE-B*27:03/Human HSP90AB1 (RRVKEVVKK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY190 | Mouse Anti-HSP90AB1 scFv-Fc Chimera (VS-0325-FY190) | WB, IP | Mouse IgG2a, scFv-Fc |
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