Mouse Anti-IDH1 Recombinant Antibody (clone 7G8A1)
CAT#: VS7-0425-WR113
This product is a mouse IgG1 recombinant antibody that binds to human IDH1. The antibody 7G8A1 recognizes a specific amino acid domain of human IDH1 (AA: 156-298). It can be used for WB, ICC, and FC assays. It cross reacts with mouse and monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62556/1538_D1_6_selected.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35248/142542_A_7_4.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35248/142542_A_6_6.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35248/142542_A_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Hep G2, RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138413-IDH1
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Specifications
- Immunogen
- Recombinant human IDH1 (AA: 156-298) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human IDH1
- Species Reactivity
- Human, Mouse, Monkey
- Clone
- 7G8A1
- Molecule Class
- Metabolic Enzyme
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Glioma (GLM)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Immunocytochemistry: 1:50 - 1:250
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
- Gene ID
- 3417
- UniProt ID
- O75874
- Long Name
- Isocitrate Dehydrogenase (Nadp(+)) 1
- Cellular Localization
- Cytoplasm; Peroxisome
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001269315.1
- Function
- Oxidoreductase
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
See other products for "IDH1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1615z | Mouse Anti-IDH1 Recombinant Antibody (clone 27C2) | WB, FC, IF, IHC | Mouse IgG1, κ |
| HPAB-0136-YC | Mouse Anti-IDH1 Recombinant Antibody (clone A2E8) | ELISA, WB, DB | Mouse IgG |
| MRO-0876-CN | Mouse Anti-IDH1 Recombinant Antibody (clone A11-B4) | WB, IHC | Mouse IgG1 |
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| VS7-0425-WR112 | Mouse Anti-IDH1 Recombinant Antibody (clone 4A4A8) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1733 | Rabbit Anti-IDH1 Recombinant Antibody (clone DS1733AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0136-YC-S(P) | Mouse Anti-IDH1 Recombinant Antibody (clone A2E8); scFv Fragment | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0136-YC-F(E) | Mouse Anti-IDH1 Recombinant Antibody (clone A2E8); Fab Fragment | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1486 | AbPlus™ Anti-IDH1 Magnetic Beads (VS-0724-YC1486) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY268 | Mouse Anti-NHP IDH1 Recombinant Antibody (clone OTI24A2) | WB, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1087 | Anti-IDH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3383 | Anti-Mouse IDH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3382 | Anti-Human IDH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3384 | Anti-Rat IDH1 Immunohistochemistry Kit | IHC |
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