Human Anti-IRF6 Recombinant Antibody (clone 2506W1331)
CAT#: VS-0625-FY1436
This recombinant antibody is a human IgG1 antibody, generated via recombinant antibody (rAb) phage display. Its specificity and affinity for the IRF6 protein have been thoroughly tested under non-denaturing conditions. This versatile antibody is validated for ELISA, Spiked IP, andIF. The antibody targets a broad epitope spanning amino acids 1-467 of the IRF6 protein, offering a comprehensive tool for detecting and analyzing IRF6 in its native conformation across diverse experimental applications.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm, cytosol & cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/1576_F9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/1685_F8_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/154587_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/154587_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/154587_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/154587_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/154587_A_6_6.jpg
Normal Tissue
Figure 8 Skin
Epidermal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranousnucl ear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63121/154587_B_5_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A-431, HaCaT, HBEC3-KT, hTCEpi, hTERT-HME1, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117595-IRF6
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The antibody reacts with human IRF6.
- Species Reactivity
- Human
- Clone
- 2506W1331
- Applications
- ELISA, Spiked Immunoprecipitation, Immunofluorescence
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (over 6 months). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IRF6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1969-sdAb | Recombinant Anti-human IRF6 VHH Single Domain Antibody | ELISA, Neut, ICC, FC | Llama VHH |
| FAMAB-0332-CN-VHH | Camelid Anti-IRF6 Recombinant Single Domain Antibody (FAMAB-0332-CN-VHH) | ELISA | Camelid VHH |
| FAMAB-0333-CN-VHH | Camelid Anti-IRF6 Recombinant Single Domain Antibody (FAMAB-0333-CN-VHH) | ELISA | Camelid VHH |
| FAMAB-0334-CN-VHH | Camelid Anti-IRF6 Recombinant Single Domain Antibody (FAMAB-0334-CN-VHH) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-356 | Recombinant Human Anti-Human IRF6 Antibody, clone U69 | IP, IF | IgG |
| MRO-0872-CN | Recombinant Rabbit Anti-IRF6 Monoclonal Antibody (CBACN-324) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ130 | Rabbit Anti-IRF6 Recombinant Antibody (VS3-CJ130) | WB, ICC, IF, IHC | Rabbit IgG |
| VS3-FY1584 | Mouse Anti-IRF6 Recombinant Antibody (clone ZN106) | IHC-P | Mouse IgG2b |
| VS-0625-FY1437 | Human Anti-IRF6 Recombinant Antibody (clone 2506W1332) | Spiked IP, IF | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1858 | Hi-Affi™ Recombinant Rabbit Anti-IRF6 Monoclonal Antibody (DS1858AB) | WB, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1956 | PE-A2/Human IRF6 (GLYPGLIWL) MHC Tetramer | FCM | |
| MHC-LC1983 | PE-A*02:01/Human IRF6 (GLYPGLIWL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1147 | Anti-IRF6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3594 | Anti-Mouse IRF6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3593 | Anti-Human IRF6 Immunohistochemistry Kit | IHC |
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