Mouse Anti-ITGA5 Recombinant Antibody (VS7-0425-WR155) (CAT#: VS7-0425-WR155)
This product is a mouse IgG recombinant antibody that binds to human ITGA5. The antibody can be used for WB and FC assays. It cross reacts with mouse, rat, and bovine.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 High expression in placenta
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/ihc_selected.jpg
Figure 2 Low expression in skeletal muscle
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_B_1_6_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_B_1_6_val_selected.jpg
Figure 3 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9008/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9008/ihc_selected.jpg
Figure 4 Liver
(Hepatocytes Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_8_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_8_4.jpg
Figure 5 Placenta
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: Low Intensity: Moderate Quantity: <25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_3_7.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: Low Intensity: Moderate Quantity: <25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_3_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1, HUVEC TERT2, TIME, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000161638-ITGA5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000161638-ITGA5
Specifications
- Immunogen
- Recombinant human ITGA5 (N-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ITGA5
- Species Reactivity
- Human, Mouse, Rat, Bovine
- Molecule Class
- Integrin/Cell Adhesion Molecule
- Applications
- WB, FC
- Conjugate
- Unconjugated
- Related Disease
- Various cancers; Fibrosis; Cardiovascular diseases
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:100 - 1:200
Target
- Alternative Names
- FNRA; CD49e; VLA-5; VLA5A
- Gene ID
- 3678
- UniProt ID
- P08648
- Long Name
- Integrin Subunit Alpha 5
- Cellular Localization
- Cell junction, Membrane
- Post Translation Modifications
- Proteolytic cleavage by PCSK5 mediates activation of the precursor.
- Protein Refseq
- NP_002196
- Function
- integrin binding; platelet-derived growth factor receptor binding; protein binding; receptor activity; vascular endothelial growth factor receptor 2 binding;
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-077 | Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody (Volociximab) | ELISA, IHC | IgG4 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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