Hi-Affi™ Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (DS1874AB)
CAT#: MOR-1874
Recombinant Rabbit anti-ITGA5 monoclonal antibody for WB, IHC-P, ICC/IF, IP, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 High expression in placenta
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/ihc_selected.jpg
Normal Tissue
Figure 2 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_B_1_6_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9008/ihc_selected.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_8_4.jpg
Normal Tissue
Figure 5 Placenta
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: Low Intensity: Moderate Quantity: <25% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2642/10675_A_3_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1, HUVEC TERT2, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000161638-ITGA5
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Specifications
- Immunogen
- Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal).
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS1874AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry (Paraffin); Immunocytochemistry/Immunofluorescence; Immunoprecipitation; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ITGA5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1727z | Mouse Anti-ITGA5 Recombinant Antibody (clone 11B1) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MRO-0849-CN | Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (CBACN-310) | WB, IF, IHC, IP, FC | Rabbit IgG |
| HPAB-0398-WJ | Human Anti-ITGA5 Recombinant Antibody (HPAB-0398-WJ) | ELISA | Humanized IgG |
| HPAB-0399-WJ | Mouse Anti-ITGA5 Recombinant Antibody (HPAB-0399-WJ) | FC | Mouse IgG |
| FAMAB-1184CQ | Mouse Anti-ITGA5 Recombinant Antibody (clone SNAKA51) | IHC, WB, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-077 | Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody (Volociximab) | ELISA, IHC | IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1554CQ | Recombinant Mouse Anti-ITGA5 Antibody (NKI-SAM-1) | FC, IP, ELISA, BL | IgG2b, κ |
| NEUT-1555CQ | Recombinant Armenian Hamster Anti-Itga5 Antibody (HMα5-1) | FC, BL, IP | IgG |
| NEUT-1556CQ | Recombinant Rat Anti-Itga5 Antibody (5H10-27) | FC, BL, IHC-Fr, FuncS | IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-077 | Afuco™ Anti-ITGA5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-077) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0398-WJ-S(P) | Human Anti-ITGA5 Recombinant Antibody; scFv Fragment (HPAB-0398-WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-0399-WJ-S(P) | Mouse Anti-ITGA5 Recombinant Antibody; scFv Fragment (HPAB-0399-WJ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0398-WJ-F(E) | Human Anti-ITGA5 Recombinant Antibody; Fab Fragment (HPAB-0398-WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-0399-WJ-F(E) | Mouse Anti-ITGA5 Recombinant Antibody; Fab Fragment (HPAB-0399-WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1485 | AbPlus™ Anti-ITGA5 Magnetic Beads (VS-0724-YC1485) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1155 | Anti-ITGA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3627 | Anti-Mouse ITGA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3629 | Anti-Human ITGA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3628 | Anti-Rat ITGA5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY136 | Rabbit Anti-ITGA5 scFv-Fc Chimera (VS-0425-FY136) | ELISA, Flow, ICC, IF, IHC, BLOCK | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC570 | Recombinant Anti-ITGA5 Vesicular Antibody, EV Displayed (VS-0425-YC570) | ELISA, FC, Cell-uptake |
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