How does the HUTS-21 clone facilitate ITGB1 detection?

We engineered this mouse recombinant antibody to specifically recognize the 130 kDa integrin β1 (ITGB1) subunit with high specificity. The rigorous purification and quality control ensure that it performs reliably in assays such as flow cytometry, providing researchers with a dependable reagent for ITGB1 detection and analysis.

What benefits does the recombinant format offer for ITGB1 research?

Our recombinant anti-ITGB1 antibody offers high purity and reproducibility, thanks to stringent SDS-PAGE and HPLC validations. Its specific binding to the integrin β1 subunit enables consistent flow cytometry data, and the clearly provided storage guidelines further facilitate its use in diverse cell adhesion research assays.

Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21)

CAT#: HPAB-0253CQ

This product is a recombinant mouse antibody that recognizes ITGB1. The antibody reacts with the 130 kDa integrin β1 subunit, CD29.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line BJ

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line BJ

Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69003/165766_B_5_7_selected.jpg

Normal Tissue Figure 3 Immunofluorescent staining of human cell line BJ

Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/ihc_selected.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_A_7_5.jpg

Normal Tissue Figure 5 Smooth muscle

Smooth muscle cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_B_6_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150093-ITGB1

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Specifications

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG2a, κ

Specificity
Human ITGB1

Species Reactivity
Human

Clone
HUTS-21

Applications
FC

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Integrin Subunit Beta 1

Gene ID
3688

UniProt ID
P05556

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Breast cancer biomarkers at key points during disease progression

Strong ITGB1 Detection

The Mouse Anti-ITGB1 recombinant antibody (clone HUTS-21) has been very effective in flow cytometry experiments. Its ability to target the 130 kDa integrin β1 subunit with high purity is clearly reflected in my results. The detailed product information and clear handling instructions made this reagent a dependable choice for my studies.

Consistent and Trustworthy

I found this anti-ITGB1 antibody to be both consistent and trustworthy for cell adhesion studies. Its excellent purity profile and specificity for the integrin β1 subunit have provided reproducible results in flow cytometry. The comprehensive guidelines on storage and use further reinforce its reliability in my experiments.

Q&As

How does the HUTS-21 clone facilitate ITGB1 detection?

A: We engineered this mouse recombinant antibody to specifically recognize the 130 kDa integrin β1 (ITGB1) subunit with high specificity. The rigorous purification and quality control ensure that it performs reliably in assays such as flow cytometry, providing researchers with a dependable reagent for ITGB1 detection and analysis.
What benefits does the recombinant format offer for ITGB1 research?

A: Our recombinant anti-ITGB1 antibody offers high purity and reproducibility, thanks to stringent SDS-PAGE and HPLC validations. Its specific binding to the integrin β1 subunit enables consistent flow cytometry data, and the clearly provided storage guidelines further facilitate its use in diverse cell adhesion research assays.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0253CQ.pdf

MSDS

MSDS - HPAB-0253CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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