CytoStream™ Rabbit Anti-ITGB1 Recombinant Antibody (VS13-YC603)
CAT#: VS13-YC603
The CytoStream™ Rabbit Anti-ITGB1 Recombinant Antibody is specific for human and mouse ITGB1. ITGB1, part of integrin family membrane receptors, involves cell adhesion and recognition across various processes like hemostasis, immune response, and tumor metastasis. It encodes a beta subunit with multiple spliced transcript variants encoding different isoforms. The anti-ITGB1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69003/165766_B_5_7_selected.jpg
Normal Tissue
Figure 3 Immunofluorescent staining of human cell line BJ
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3434/ihc_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_A_7_5.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59297/139504_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150093-ITGB1
❮
❯
Specifications
- Immunogen
- Recombinant human Integrin beta-1, aa 652-701.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ITGB1
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Immunohistochemistry-paraffin (IHC-P): 1/50
Flow cytometry (FC): 1/50
Target
- Alternative Names
- Integrin Subunit Beta 1
- Gene ID
- 3688
- UniProt ID
- P05556
- Long Name
- Integrin Subunit Beta 1
- Sequence Similarities
- Belongs to the integrin beta chain family.
- Cellular Localization
- Cell membrane; Cell projection; Cleavage furrow
- Protein Refseq
- NP_002202.2; NP_391988.1; NP_596867.1
- Function
- Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity).
Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099).
ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).
ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973).
Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS13-YC603. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ITGB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-772LC | Anti-human ITGB1 Recombinant Antibody (1B4) (TAB-772LC) | FuncS | |
| TAB-772LC-S(P) | Anti-human ITGB1 scFv Fragment (1B4) | ELISA | |
| TAB-772LC-F(E) | Anti-human ITGB1 Fab Fragment (1B4) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0243CT | Recombinant Mouse anti-Human ITGB1 Monoclonal antibody (23H20) | ELISA, FC, ICC, IF, WB | |
| VS3-XY926 | Mouse Anti-ITGB1 Recombinant Antibody, FITC (clone 4ATS2/16.2.1) | FC, IF | Mouse IgG1 |
| VS3-XY927 | Mouse Anti-ITGB1 Recombinant Antibody, PE (clone 4ATS2/16.2.1) | FC, IF | Mouse IgG1 |
| VS3-XY928 | Mouse Anti-ITGB1 Recombinant Antibody (clone 4ATS2/16.2.1) | FC, IF | Mouse IgG1 |
| VS3-XY933 | Mouse Anti-ITGB1 Recombinant Antibody (clone 3B6G3) | IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0277MZ | Chicken Anti-Integrin β1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1585CQ | Recombinant Mouse Anti-ITGB1 Antibody (CBL252) | FC, ELISA, WB, IP, FuncS, Inhib | IgG1 |
| NEUT-1587CQ | Recombinant Mouse Anti-ITGB1 Antibody (P5D2) | BL, FC, IP, CyTOF, ICC | IgG1 |
| NEUT-1588CQ | Recombinant Armenian Hamster Anti-Itgb1 Antibody (HMβ1-1) | FC, IP, BL, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1586CQ | Recombinant Mouse Anti-ITGB1 Antibody (P4C10) | WB, Neut, ELISA, FC, ICC, IF, IHC-Fr, IP | IgG1 |
| NEUT-1589CQ | Recombinant Armenian hamster Anti-Itgb1 Antibody (HMb1-1) | FC, FuncS, Neut | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1882 | Hi-Affi™ Recombinant Rabbit Anti-ITGB1 Monoclonal Antibody (DS1882AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN | Human Anti-ITGB1 Recombinant Antibody | ELISA, WB, IHC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN-F(E) | Human Anti-ITGB1 Recombinant Antibody; Fab Fragment | ELISA, WB, IHC | Human Fab |
| HPAB-0253CQ-F(E) | Mouse Anti-ITGB1 Recombinant Antibody (clone HUTS-21); Fab Fragment | FC | Mouse Fab |
| HPAB-0254CQ-F(E) | Rat Anti-ITGB1 Recombinant Antibody (clone MAb AIIB2); Fab Fragment | ELISA, FC, WB, ICC, Block, IP, IHC | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-047CN-S(P) | Human Anti-ITGB1 Recombinant Antibody; scFv Fragment | ELISA, WB, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY161 | AbPlus™ Anti-ITGB1 Magnetic Beads (P4C10) | IP, Protein Purification | |
| VS-0724-YC1500 | AbPlus™ Anti-Itgb1 Magnetic Beads (VS-0724-YC1500) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC53 | Rabbit Anti-ITGB1 Antibody (VS-0924-YC53) - Cancer Stem Cell Marker | IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY289 | Mouse Anti-NHP ITGB1 Recombinant Antibody (clone 3B6) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY152 | CytoStream™ Mouse Anti-ITGB1 Recombinant Antibody (VS-0225-XY152) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1156 | Anti-ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3653 | Anti-Mouse ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3655 | Anti-Human ITGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3654 | Anti-Porcine ITGB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC597 | Recombinant Anti-ITGB1 Vesicular Antibody, EV Displayed (VS-0425-YC597) | Block, ELISA, FC, Cell-uptake |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
-2-1.png)
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, WB, BLI, SPR
Application: WB, ELISA, FuncS
-YJ-3.jpg)
Application: ELISA
Application: IHC, ELISA, FC, WB, ADCC, FuncS
-2-1.png)
Application: ELISA
Application: Block, IP, IF, FC
Application: ELISA, PD, Activ, PK, Stim
-2.png)
Application: WB, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
