Mouse Anti-JAK3 Recombinant Antibody (clone 6B5)
CAT#: VS3-WK1605
This antibody is a mouse recombinant antibody against human JAK3, which is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. The clone 6B5 can be used in IHC analysis at a dilution of 1/50-1/200.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70314/1813_H2_9_cr59f2f211a23e0_selected.jpg
Normal Tissue
Figure 2 Appendix
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70314/154739_A_3_2_val_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: Low Intensity: Weak Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70314/154739_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70314/154739_A_7_8.jpg
Normal Tissue
Figure 5 Tonsil
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70314/154739_A_4_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HEL, HMC-1, Karpas-707, U-266/70, U-266/84, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105639-JAK3
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Specifications
- Immunogen
- Synthesized peptide derived from human JAK3
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human JAK3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 6B5
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC: 1/50-1/200
Target
- Alternative Names
- JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
- Gene ID
- 3718
- UniProt ID
- P52333
- Long Name
- Janus kinase 3
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).
- Protein Refseq
- NP_000206.2
- Function
- Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "JAK3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1348z | Mouse Anti-JAK3 Recombinant Antibody (clone 30G11) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MRO-0882-CN | Recombinant Rabbit Anti-JAK3 Monoclonal Antibody (JM66-31) | WB, IHC, IF | Rabbit IgG |
| ZG-0781J | Rabbit Anti-JAK3 Recombinant Antibody (clone 1C7) | ELISA, IHC | Rabbit IgG |
| VS3-XY949 | Mouse Anti-JAK3 Recombinant Antibody (clone 5H2) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS7-0425-WR168 | Mouse Anti-JAK3 Monoclonal Antibody (VS7-0425-WR168) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1899 | Hi-Affi™ Recombinant Rabbit Anti-JAK3 Monoclonal Antibody (DS1899AB) | FC, IP, WB | IgG |
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