Rabbit Anti-KEAP1 Recombinant Antibody (VS3-FY2749)
CAT#: VS3-FY2749
The anti-KEAP1 antibody is specifically created to bind human Keap1 protein. It exhibits cross-reactivity with mouse. This monoclonal antibody proves valuable in disease research related to Goiter, Multinodular 1, with or without Sertoli-Leydig Cell Tumors and Squamous Cell Carcinoma.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5558/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5558/15846_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079999-KEAP1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Kelch like ECH associated protein 1 of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC
- Conjugate
- Unconjugated, FITC
- Related Disease
- Goiter, Multinodular 1, With Or Without Sertoli-Leydig Cell Tumors and Squamous Cell Carcinoma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Kelch Like ECH Associated Protein 1; Kelch-Like Family Member 19; Cytosolic Inhibitor Of Nrf2; Kelch-Like Protein 19; KLHL19; INrf2; Kelch-Like ECH-Associated Protein 1; KIAA0132
- Gene ID
- 9817
- UniProt ID
- Q14145
- Sequence Similarities
- Belongs to the KEAP1 family.
- Cellular Localization
- Cytoplasm
Nucleus
- Post Translation Modifications
- Degraded via a proteasomal-independent process during selective autophagy: interaction with phosphorylated SQSTM1/p62 sequesters KEAP1 in inclusion bodies, leading to its degradation.
Auto-ubiquitinated by the BCR(KEAP1) complex.
Quinone-induced oxidative stress, but not sulforaphane, increases its ubiquitination.
Ubiquitination and subsequent degradation is most pronounced following prolonged exposure of cells to oxidative stress, particularly in glutathione-deficient cells that are highly susceptible to oxidative stress.
Ubiquitination at Lys61, Lys84, Lys108, Lys287, Lys312, and Lys615
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 11 sites, 1 O-linked glycan (11 sites)
- Protein Refseq
- NP_036421.2; NP_987096.1
- Function
- Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed:14585973, 15379550, 15572695, 15983046, 15601839).
KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed:15601839, 16006525).
In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KEAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4068z | Mouse Anti-KEAP1 Recombinant Antibody (clone 22D2) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| VS3-XY966 | Mouse Anti-KEAP1 Recombinant Antibody (clone 1F10B6) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS3-XY967 | Mouse Anti-KEAP1 Recombinant Antibody (clone 7G4B10) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS13-YC618 | Rabbit Anti-KEAP1 Recombinant Antibody (VS13-YC618) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1928 | Hi-Affi™ Recombinant Rabbit Anti-KEAP1 Monoclonal Antibody (DS1928AB) | WB, ICC, IF, FC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1181 | Anti-KEAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3762 | Anti-Mouse KEAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3761 | Anti-Human KEAP1 Immunohistochemistry Kit | IHC |
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