Rabbit Anti-KEAP1 Recombinant Antibody (VS3-FY2749) (CAT#: VS3-FY2749)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Kelch like ECH associated protein 1 of human and mouse origins

• Species Reactivity
• Human, Mouse

• Applications
• WB, IHC

• Conjugate
• Unconjugated, FITC

• Related Disease
• Goiter, Multinodular 1, With Or Without Sertoli-Leydig Cell Tumors and Squamous Cell Carcinoma

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• Kelch Like ECH Associated Protein 1; Kelch-Like Family Member 19; Cytosolic Inhibitor Of Nrf2; Kelch-Like Protein 19; KLHL19; INrf2; Kelch-Like ECH-Associated Protein 1; KIAA0132

• Gene ID
• 9817

• UniProt ID
• Q14145

• Sequence Similarities
• Belongs to the KEAP1 family.

• Cellular Localization
• Cytoplasm
  Nucleus

• Post Translation Modifications
• Degraded via a proteasomal-independent process during selective autophagy: interaction with phosphorylated SQSTM1/p62 sequesters KEAP1 in inclusion bodies, leading to its degradation.
  Auto-ubiquitinated by the BCR(KEAP1) complex.
  Quinone-induced oxidative stress, but not sulforaphane, increases its ubiquitination.
  Ubiquitination and subsequent degradation is most pronounced following prolonged exposure of cells to oxidative stress, particularly in glutathione-deficient cells that are highly susceptible to oxidative stress.
  Ubiquitination at Lys61, Lys84, Lys108, Lys287, Lys312, and Lys615
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyGen 11 sites, 1 O-linked glycan (11 sites)

• Protein Refseq
• NP_036421.2; NP_987096.1

• Function
• Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed:14585973, 15379550, 15572695, 15983046, 15601839).
  KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed:15601839, 16006525).
  In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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