Rabbit Anti-KLF4 Recombinant Antibody (clone R03-5H7) (CAT#: VS3-XY3408)
This is a primary rabbit antibody recognizing with human KLF4 (KLF transcription factor 4), which regulates the expression of key transcription factors during embryonic development. KLF4 plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. This antibody also has cross-reactivity against mouse and rat KLF4. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
Figure 2 High expression in esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat KLF4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5H7
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- EZF; GKLF
- Gene ID
- 9314
- UniProt ID
- O43474
- Long Name
- KLF transcription factor 4
- Cellular Localization
- Nucleus, Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys32 (NX_O43474 [NX_O43474-3])
Modification sites at PhosphoSitePlus (O43474)
Glycosylation from GlyGen (O43474) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_004226.3; NP_001300981.1
- Function
- Transcription factor; can act both as activator and as repressor.
Binds the 5'-CACCC-3' core sequence.
Binds to the promoter region of its own gene and can activate its own transcription.
Regulates the expression of key transcription factors during embryonic development.
Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Cancer Stem Cell Biomarker Specific Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3037z | Mouse Anti-KLF4 Recombinant Antibody (clone 36H2) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| VS3-CJ33 | Mouse Anti-KLF4 Recombinant Antibody (VS3-CJ33) | WB, ELISA | Mouse IgG |
| VS3-XY980 | Mouse Anti-KLF4 Recombinant Antibody (clone 1E5) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY798 | Recombinant Rabbit Anti-Klf4 Antibody (clone R03-2I8) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC626 | Rabbit Anti-KLF4 Recombinant Antibody (VS13-YC626) | WB, ICC, IF, IHC-P | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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