Rabbit Anti-KLF4 Recombinant Antibody (clone R03-5H7)
CAT#: VS3-XY3408
This is a primary rabbit antibody recognizing with human KLF4 (KLF transcription factor 4), which regulates the expression of key transcription factors during embryonic development. KLF4 plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. This antibody also has cross-reactivity against mouse and rat KLF4. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
Normal Tissue
Figure 2 High expression in esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat KLF4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5H7
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- EZF; GKLF
- Gene ID
- 9314
- UniProt ID
- O43474
- Long Name
- KLF transcription factor 4
- Cellular Localization
- Nucleus, Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys32 (NX_O43474 [NX_O43474-3])
Modification sites at PhosphoSitePlus (O43474)
Glycosylation from GlyGen (O43474) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_004226.3; NP_001300981.1
- Function
- Transcription factor; can act both as activator and as repressor.
Binds the 5'-CACCC-3' core sequence.
Binds to the promoter region of its own gene and can activate its own transcription.
Regulates the expression of key transcription factors during embryonic development.
Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KLF4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1956 | Hi-Affi™ Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (DS1956AB) | ELISA, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0901-CN | Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (JF98-08) | WB, IF, IHC | Rabbit IgG |
| ZG-0387J | Mouse Anti-KLF4 Recombinant Antibody (ZG-0387J) | WB, IHC, ELISA | Mouse IgG |
| ZG-0388J | Mouse Anti-KLF4 Recombinant Antibody (ZG-0388J) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0786J | Rabbit Anti-KLF4 Recombinant Antibody (clone 3A1) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-QX641 | Mouse Anti-KLF4 Recombinant Antibody (clone 56CT5.1.6) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC96 | AbPlus™ Anti-KLF4 Magnetic Beads (VS-0724-YC96) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC36 | Mouse Anti-KLF4 Recombinant Antibody (VS-0924-YC36) - Cancer Stem Cell Marker | IHC, WB, IF | |
| VS-0924-YC63 | Rabbit Anti-KLF4 Antibody (VS-0924-YC63) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3795 | Anti-KLF4 Immunohistochemistry Kit | IHC |
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