Rabbit Anti-L1CAM Antibody (VS-0924-YC65) - Cancer Stem Cell Marker
CAT#: VS-0924-YC65
This product is an anti-L1CAM antibody (VS-0924-YC65). It specifically targets the human L1CAM protein, a marker for cancer stem cells (CSCs). The antibody is suitable for detecting L1CAM in CSC tissues or cells using immunohistochemistry, at a dilution range of 1/200-1/500.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/208_G1_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/207_G1_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/206_G1_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, RH-30, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198910-L1CAM
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human L1CAM
- Species Reactivity
- Human
- Applications
- IHC
- Related Disease
- Disease variant, Hereditary spastic paraplegia, Hirschsprung disease, Intellectual disability, Neurodegeneration
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/200-1/500
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0398-CN-F(E) | Mouse Anti-L1CAM Recombinant Antibody; Fab Fragment (HPAB-0398-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-J0160-YC-F(E) | Human Anti-L1CAM Recombinant Antibody (clone Ab4); Fab Fragment | ELISA, FC, WB, FuncS | Human Fab |
| HPAB-0561-YJ-F(E) | Mouse Anti-L1CAM Recombinant Antibody (clone A10-A3); Fab Fragment | ELISA, FC, FuncS | Mouse Fab |
| HPAB-0562-YJ-F(E) | Human Anti-L1CAM Recombinant Antibody (clone cA10-A3); Fab Fragment | ELISA, FC, FuncS | Chimeric (mouse/human) Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0191-YC-S(P) | Human Anti-L1CAM Recombinant Antibody (clone G6); scFv Fragment | ELISA, IF, WB | Human scFv |
| FAMAB-0192-YC-S(P) | Human Anti-L1CAM Recombinant Antibody (clone H2); scFv Fragment | ELISA, IF, WB | Human scFv |
| HPAB-0561-YJ-S(P) | Mouse Anti-L1CAM Recombinant Antibody (clone A10-A3); scFv Fragment | ELISA, FC, FuncS | Mouse scFv |
| HPAB-0562-YJ-S(P) | Mouse Anti-L1CAM Recombinant Antibody (clone cA10-A3); scFv Fragment | ELISA, FC, FuncS | Mouse scFv |
| HPAB-0563-YJ-S(P) | Human Anti-L1CAM Recombinant Antibody (clone HzA10A3-2.1); scFv Fragment | ELISA, FC, FuncS | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1223 | Anti-L1CAM Immunohistochemistry Kit | IHC | |
| VS-0525-XY3907 | Anti-Mouse L1CAM Immunohistochemistry Kit | IHC | |
| VS-0525-XY3906 | Anti-Human L1CAM Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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