Anti-Human L1CAM Recombinant Antibody (L1-9.3)

Name: Anti-Human L1CAM Recombinant Antibody (L1-9.3)
SKU: TAB-0636CL

CAT#: TAB-0636CL

This recombinant monoclonal antibody L1-9.3 is a mouse monoclonal antibody that specifically binds to human L1CAM. It is recommended for the detection of human L1CAM by ELISA, WB, IHC, and FC.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/208_G1_2_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/207_G1_1_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/206_G1_1_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuropil
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_B_8_5.jpg

Normal Tissue Figure 5 Colon

Endothelial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%

Peripheral nerve/ganglion
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_7_3.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts
Staining:

High

Intensity: Strong
Quantity:>75%
Distal tubules
Staining:

High

Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10896/27469_A_8_5.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, RH-30, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198910-L1CAM

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Specifications

Host Species
Mouse

Antibody Isotype
IgG

Specificity
Human

Clone
L1-9.3

Applications
WB, IHC, ELISA, FC

Related Disease
Tumorigenic disease

Applications

Application Notes
The L1CAM antibody has been reported in applications of Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Flow Cytometry.

Target

Alternative Names
L1CAM; L1 cell adhesion molecule; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1; neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1

Gene ID
3897

UniProt ID
P32004

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Breast cancer biomarkers at key points during disease progression

S***h

Excellent for WB

As someone deeply engaged in neural cancer research, the Anti-Human L1CAM Recombinant Antibody (L1-9.3) has been a remarkable tool. In WB experiments, using lysates from neuroblastoma cells as the antigen, I incubated the antibody at room temperature for two hours. It accurately identified L1CAM, with distinct bands appearing, facilitating my research on neuroblastoma progression.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-0636CL, RRID: AB_3714272)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-0636CL.pdf

MSDS

MSDS - TAB-0636CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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